Leishmaniinae: Evolutionary inferences based on protein expression profiles (PhyloQuant) congruent with phylogenetic relationships among Leishmania, Endotrypanum, Porcisia, Zelonia, Crithidia, and Leptomonas.

Autor: Mule SN; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Alemán EV; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Rosa-Fernandes L; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Saad JS; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., de Oliveira GS; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Martins D; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Angeli CB; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Brandt-Almeida D; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Cortez M; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Larsen MR; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK, Denmark., Shaw JJ; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Teixeira MMG; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Palmisano G; Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2024 Sep; Vol. 24 (18), pp. e2100313. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1002/pmic.202100313
Abstrakt: Evolutionary relationships among parasites of the subfamily Leishmaniinae, which comprises pathogen agents of leishmaniasis, were inferred based on differential protein expression profiles from mass spectrometry-based quantitative data using the PhyloQuant method. Evolutionary distances following identification and quantification of protein and peptide abundances using Proteome Discoverer and MaxQuant software were estimated for 11 species from six Leishmaniinae genera. Results clustered all dixenous species of the genus Leishmania, subgenera L. (Leishmania), L. (Viannia), and L. (Mundinia), sister to the dixenous species of genera Endotrypanum and Porcisia. Placed basal to the assemblage formed by all these parasites were the species of genera Zelonia, Crithidia, and Leptomonas, so far described as monoxenous of insects although eventually reported from humans. Inferences based on protein expression profiles were congruent with currently established phylogeny using DNA sequences. Our results reinforce PhyloQuant as a valuable approach to infer evolutionary relationships within Leishmaniinae, which is comprised of very tightly related trypanosomatids that are just beginning to be phylogenetically unraveled. In addition to evolutionary history, mapping of species-specific protein expression is paramount to understand differences in infection processes, tissue tropisms, potential to jump from insects to vertebrates including humans, and targets for species-specific diagnostic and drug development.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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