Nitrogen stable isotope analysis of sulfonamides by derivatization-gas chromatography-isotope ratio mass spectrometry.

Autor: Dou Q; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Canavan A; Chair of Analytical Chemistry and Water Chemistry, School of Natural Sciences, Department of Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748, Garching, Germany., Fu Y; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Xiang L; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Wang Y; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Wang X; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Jiang X; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China.; University of Chinese Academy of Science, Beijing, 100049, China., Dirr C; Chair of Analytical Chemistry and Water Chemistry, School of Natural Sciences, Department of Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748, Garching, Germany., Wang F; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, No. 71 East Beijing Road, Nanjing, 210008, China. wangfang@issas.ac.cn.; University of Chinese Academy of Science, Beijing, 100049, China. wangfang@issas.ac.cn., Elsner M; Chair of Analytical Chemistry and Water Chemistry, School of Natural Sciences, Department of Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748, Garching, Germany. m.elsner@tum.de.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Aug; Vol. 416 (19), pp. 4237-4247. Date of Electronic Publication: 2024 Jun 07.
DOI: 10.1007/s00216-024-05361-2
Abstrakt: The continuous introduction of micropollutants into the environment through livestock farming, agricultural practices, and wastewater treatment is a major concern. Among these pollutants are synthetic sulfonamide antibiotics such as sulfamethoxazole, which are not always fully degraded and pose a risk of fostering antimicrobial resistance. It is challenging to assess the degradation of sulfonamides with conventional concentration measurements. This study introduces compound-specific isotope analysis of nitrogen isotope ratios at natural abundances by derivatization-gas chromatography hyphenated with isotope ratio mass spectrometry (derivatization-GC-IRMS) as a new and more precise method for tracing the origin and degradation of sulfonamides. Here, sulfamethoxazole was used as a model compound to develop and optimize the derivatization conditions using (trimethylsilyl)diazomethane as a derivatization reagent. With the optimized conditions, accurate and reproducible δ 15 N analysis of sulfamethoxazole by derivatization-GC-IRMS was achieved in two different laboratories with a limit for precise isotope analysis of 3 nmol N on column, corresponding to 0.253 µg non-derivatized SMX. Application of the method to four further sulfonamides, sulfadiazine, sulfadimethoxine, sulfadimidine, and sulfathiazole, shows the versatility of the developed method. Its benefit was demonstrated in a first application, highlighting the possibility of distinguishing sulfamethoxazole from different suppliers and pharmaceutical products.
(© 2024. The Author(s).)
Databáze: MEDLINE
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