The endophytic fungus Serendipita indica affects auxin distribution in Arabidopsis thaliana roots through alteration of auxin transport and conjugation to promote plant growth.

Autor:	González Ortega-Villaizán A; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain., King E; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain., Patel MK; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain., Pérez-Alonso MM; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain., Scholz SS; Department of Plant Physiology, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Friedrich-Schiller-University Jena, Jena, Germany., Sakakibara H; RIKEN Center for Sustainable Resource Science, Yokohama, Japan.; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan., Kiba T; RIKEN Center for Sustainable Resource Science, Yokohama, Japan.; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan., Kojima M; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Takebayashi Y; RIKEN Center for Sustainable Resource Science, Yokohama, Japan., Ramos P; Instituto de Ciencias Biológicas, Campus Talca, Universidad de Talca, Talca, Chile., Morales-Quintana L; Multidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Universidad Autonóma de Chile, Talca, Chile., Breitenbach S; Institute of Botany, Technische Universität Dresden, Dresden, Germany., Smolko A; Department for Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia., Salopek-Sondi B; Department for Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia., Bauer N; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia., Ludwig-Müller J; Institute of Botany, Technische Universität Dresden, Dresden, Germany., Krapp A; INRAE, AgroParisTech, Institut Jean-Pierre Bourgin, Université Paris-Saclay, Versailles, France., Oelmüller R; Department of Plant Physiology, Matthias Schleiden Institute of Genetics, Bioinformatics and Molecular Botany, Friedrich-Schiller-University Jena, Jena, Germany., Vicente-Carbajosa J; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain.; Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), Madrid, Spain., Pollmann S; Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA/CSIC), Campus de Montegancedo, Madrid, Spain.; Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Plant, cell & environment [Plant Cell Environ] 2024 Oct; Vol. 47 (10), pp. 3899-3919. Date of Electronic Publication: 2024 Jun 07.
DOI:	10.1111/pce.14989
Abstrakt:	Plants share their habitats with a multitude of different microbes. This close vicinity promoted the evolution of interorganismic interactions between plants and many different microorganisms that provide mutual growth benefits both to the plant and the microbial partner. The symbiosis of Arabidopsis thaliana with the beneficial root colonizing endophyte Serendipita indica represents a well-studied system. Colonization of Arabidopsis roots with S. indica promotes plant growth and stress tolerance of the host plant. However, until now, the molecular mechanism by which S. indica reprograms plant growth remains largely unknown. This study used comprehensive transcriptomics, metabolomics, reverse genetics, and life cell imaging to reveal the intricacies of auxin-related processes that affect root growth in the symbiosis between A. thaliana and S. indica. Our experiments revealed the sustained stimulation of auxin signalling in fungus infected Arabidopsis roots and disclosed the essential role of tightly controlled auxin conjugation in the plant-fungus interaction. It particularly highlighted the importance of two GRETCHEN HAGEN 3 (GH3) genes, GH3.5 and GH3.17, for the fungus infection-triggered stimulation of biomass production, thus broadening our knowledge about the function of GH3s in plants. Furthermore, we provide evidence for the transcriptional alteration of the PIN2 auxin transporter gene in roots of Arabidopsis seedlings infected with S. indica and demonstrate that this transcriptional adjustment affects auxin signalling in roots, which results in increased plant growth. (© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Databáze:	MEDLINE
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