Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron ( Crocus sativus L.).

Autor: Kalia D; Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, H.P. 176061 India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India., Jose-Santhi J; Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, H.P. 176061 India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India., Sheikh FR; Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, H.P. 176061 India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India., Singh D; Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, H.P. 176061 India., Singh RK; Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, H.P. 176061 India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India.
Jazyk: angličtina
Zdroj: Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2024 May; Vol. 30 (5), pp. 749-755. Date of Electronic Publication: 2024 May 20.
DOI: 10.1007/s12298-024-01459-0
Abstrakt: Several limitations in genetic engineering interventions in saffron exist, hindering the development of genetically modified varieties and the widespread application of genetic engineering in this crop. Lack of genome sequence information, the complexity of genetic makeup, and lack of well-established genetic transformation protocols limit its in planta functional validation of genes that would eventually lead toward crop optimization. In this study, we demonstrate agro infiltration in leaves of adult plants and whole corm before sprouting are suitable for transient gene silencing in saffron using Tobacco Rattle Virus (TRV) based virus-induced gene silencing (VIGS) targeting phytoene desaturase (PDS). Silencing of PDS resulted in bleached phenotype in leaves in both methods. TRV-mediated VIGS could be attained in saffron leaves and corms, providing an opportunity for functional genomics studies in this expensive spice crop.
Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01459-0.
Competing Interests: Conflict of interestThe authors have no relevant financial or non-financial interests to disclose.
(© Prof. H.S. Srivastava Foundation for Science and Society 2024.)
Databáze: MEDLINE
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