Spatio-temporal expression of polyphenol oxidase unveils the dynamics of L-DOPA accumulation in faba bean ( Vicia faba L.).

Autor: Jena S; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India.; The Graduate School, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India., Sanyal R; National Institute for Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067 India., Jawed DM; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India., Sengupta K; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India.; Division of Agricultural Biotechnology, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata, 700103 India., Pradhan B; Division of Agricultural Biotechnology, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata, 700103 India., Sinha SK; ICAR-National Institute of Plant Biotechnology, New Delhi, 110012 India., Sarkar B; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India., Kumar S; ICAR-National Institute of Secondary Agriculture, Ranchi, Jharkhand 834010 India., Lenka SK; Department of Plant Biotechnology, Gujarat Biotechnology University, Gandhinagar, Gujarat 382355 India., Naskar S; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India., Bhadana VP; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India., Bishi SK; ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand 834003 India.
Jazyk: angličtina
Zdroj: Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2024 May; Vol. 30 (5), pp. 839-850. Date of Electronic Publication: 2024 Apr 29.
DOI: 10.1007/s12298-024-01449-2
Abstrakt: Faba bean ( Vicia faba L.) is a winter season grain legume and a rich source of the anti-parkinson drug, L-3,4-dihydroxyphenylalanine (L-DOPA). The biosynthesis of L-DOPA in plants is not uniform and remains largely unexplored. While the hydroxylase activities of Tyrosine Hydroxylase (TH), the Cytochrome P450 (CYP450) class of enzymes, and Polyphenol Oxidases (PPOs) on tyrosine substrate have been reported in plants, only the roles of PPOs in L-DOPA biosynthesis have been recently established in velvet bean ( Mucuna pruriens ). To understand the differential accumulation of L-DOPA in different tissues of faba bean, profiling of L-Tyrosine, L-DOPA, Tyramine, and Dopamine in different tissues was performed. Differential accumulation of L-DOPA depended on tissue type and maturity. Furthermore, dopamine biosynthesis through L-DOPA from L-Tyr was confirmed in faba bean. The expression analysis of PPOs in leaf and flower tissues revealed the selective induction of only four ( HePPO-2 , HePPO-7 , HePPO-8b , and HePPO-10 ) out of ten genes encoding different PPOs mined from the faba bean genome. Higher accumulation of L-DOPA in young leaves and flower buds than in mature leaves and flowers was accompanied by significantly higher expression of HePPO-10 and HePPO-7 , respectively. The role of various transcription factors contributing to such metabolite dynamics was also predicted. Further exploration of this mechanism using a multi-omics approach can provide meaningful insight and pave the way for enhancing L-DOPA content in crops.
Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-024-01449-2.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
(© Prof. H.S. Srivastava Foundation for Science and Society 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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