Streptococcus dentisani inhibits the growth of Candida albicans and Candida glabrata: in vitro assay.

Autor: Medina JGS; Department of Microbiology, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava No. 2, Col Universitaria, 78290, San Luis Potosí, S.L.P., Mexico., Camacho JLC; Faculty of Science, Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico., Ruiz Garcia J; Laboratory of Biological Physics, Physics Institute, Autonomous University of San Luis Potosí, San Luis Potosí, México., Mira A; Department of Health and Genomics, Oral Microbiome Lab, FISABIO Foundation, Valencia, Spain., Martínez Martínez RE; Periodontics Postgraduate Program, Faculty of Dentistry, Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico., Comas-García M; Department of Sciences, Research Center for Health Sciences and Biomedicine (CICSaB), Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico., Rangel AG; Periodontics Postgraduate Program, Faculty of Dentistry, Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico., Pozos-Guillén A; Basic Sciences Laboratory, Faculty of Dentistry, Autonomous University of San Luis Potosí, San Luis Potosí, S.L.P., Mexico., Romo SA; Department of Microbiology, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava No. 2, Col Universitaria, 78290, San Luis Potosí, S.L.P., Mexico. sarayaranda@fest.uaslp.mx.; Periodontics Postgraduate Program, Faculty of Dentistry, Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico. sarayaranda@fest.uaslp.mx.; Diagnostic Clinic Dentistry School, Autonomous University of San Luis Potosí, San Luis Potosí, S.L.P., Mexico. sarayaranda@fest.uaslp.mx.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Jun 07. Date of Electronic Publication: 2024 Jun 07.
DOI: 10.1007/s10123-024-00525-7
Abstrakt: Background: Probiotic bacteria inhibit aggregation, biofilm formation, and dimorphism of Candida spp. However, the effects of a new probiotic, Streptococcus dentisani, on the growth of Candida albicans and Candida glabrata biofilms are unknown.
Objective: To determine the effect of S. dentisani on the different phases of C. albicans and C. glabrata biofilm development.
Methods: Growth quantification and ultrastructural analyses were performed on biofilms of C. albicans ATCC 90028, C. glabrata ATCC 2001, and clinical isolates of C. albicans from oral candidiasis (CA-C1), caries (CA-CR1), and periodontal pocket (CA-P1) treated with cell suspensions of S. dentisani CECT 7746. Cell viability was determined by quantifying colony-forming units (CFU/mL). The ultrastructural analyses were done with atomic force microscopy.
Results: S. dentisani induced a significant reduction (p < 0.05) of CFU/mL of immature and mature biofilm in all strains of C. albicans and C. glabrata. Microscopic analysis revealed that S. dentisani reduced C. albicans density in mixed biofilm. The fungus-bacteria interaction affected cell membrane integrity in yeast.
Conclusion: For the first time, our data elucidate the antifungal effect of S. dentisani on the development of C. albicans and C. glabrata biofilms, supporting its usefulness as a niche-specific probiotic to prevent and treat oral dysbiosis.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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