Olfactory investigation in the home cage.

Autor: Fink AJP; Present affiliation: Department of Neurobiology, Northwestern University, Evanston, IL, United States. Electronic address: af2243@columbia.edu., Hogan M; Mortimer B. Zuckerman Mind Brain Behavior Institute, Department of Neuroscience, Columbia University, New York, NY, United States., Schoonover CE; Present affiliation: Allen Institute for Neural Dynamics, Seattle, WA, United States. Electronic address: ces2001@columbia.edu.
Jazyk: angličtina
Zdroj: Neurobiology of learning and memory [Neurobiol Learn Mem] 2024 Sep; Vol. 213, pp. 107951. Date of Electronic Publication: 2024 Jun 04.
DOI: 10.1016/j.nlm.2024.107951
Abstrakt: We have developed a behavioral paradigm to study volitional olfactory investigation in mice over several months. We placed odor ports in the wall of a standard cage that administer a neutral odorant stimulus when a mouse pokes its nose inside. Even though animals were fed and watered ad libitum, and sampling from the port elicited no outcome other than the delivery of an odor, mice readily sampled these stimuli hundreds of times per day. This self-paced olfactory investigation persisted for weeks with only modest habituation following the first day of exposure to a given set of odorants. If an unexpected odorant stimulus was administered at the port, the sampling rate increased transiently (in the first 20 min) by an order of magnitude and remained higher than baseline throughout the subsequent day, indicating learned implicit knowledge. Thus, this system may be used to study naturalistic olfactory learning over extended time scales outside of conventional task structures.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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