Transcriptome responses of Arabidopsis to necrotrophic fungus Alternaria brassicae reveal pathways and candidate genes associated with resistance.

Autor: Hamsa S; Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India., Rajarammohan S; Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.; Agricultural Biotechnology Division, National Agri-Food Biotechnology Institute, SAS Nagar, Mohali, Punjab, India., Aswal M; Department of Biophysics, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India., Kumar M; Department of Biophysics, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India., Kaur J; Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India. jagreet@south.du.ac.in.; Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. jagreet@south.du.ac.in.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2024 Jun 06; Vol. 114 (3), pp. 68. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1007/s11103-024-01453-w
Abstrakt: Alternaria leaf blight (ALB), caused by a necrotrophic fungus Alternaria brassicae is a serious disease of oleiferous Brassicas resulting in significant yield losses worldwide. No robust resistance against A. brassicae has been identified in the Brassicas. Natural accessions of Arabidopsis show a spectrum of responses to A. brassicae ranging from high susceptibility to complete resistance. To understand the molecular mechanisms of resistance/ susceptibility, we analysed the comparative changes in the transcriptome profile of Arabidopsis accessions with contrasting responses- at different time points post-infection. Differential gene expression, GO enrichment, pathway enrichment, and weighted gene co-expression network analysis (WGCNA) revealed reprogramming of phenylpropanoid biosynthetic pathway involving lignin, hydroxycinnamic acids, scopoletin, anthocyanin genes to be highly associated with resistance against A. brassicae. T-DNA insertion mutants deficient in the biosynthesis of coumarin scopoletin exhibited enhanced susceptibility to A. brassicae. The supplementation of scopoletin to medium or exogenous application resulted in a significant reduction in the A. brassicae growth. Our study provides new insights into the transcriptome dynamics in A. brassicae-challenged Arabidopsis and demonstrates the involvement of coumarins in plant immunity against the Brassica pathogen A. brassicae.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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