Porous Semiconducting Polymer Nanoparticles as Intracellular Biophotonic Mediators to Modulate the Reactive Oxygen Species Balance.

Autor: Criado-Gonzalez M; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain., Marzuoli C; Center for Nano Science and Technology@PoliMi, Istituto Italiano di Tecnologia, Via Raffaele Rubattino 81, 20134 Milano, Italy.; Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, 20133 Milano, Italy., Bondi L; Department of Physics and Astronomy, University of Bologna, Viale Carlo Berti Pichat 6/2, 40127 Bologna, Italy., Gutierrez-Fernandez E; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; XMaS/BM28-ESRF, 71 Avenue Des Martyrs, F-38043 Grenoble Cedex, France.; Department of Physics, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, U.K., Tullii G; Center for Nano Science and Technology@PoliMi, Istituto Italiano di Tecnologia, Via Raffaele Rubattino 81, 20134 Milano, Italy., Lagonegro P; Center for Nano Science and Technology@PoliMi, Istituto Italiano di Tecnologia, Via Raffaele Rubattino 81, 20134 Milano, Italy., Sanz O; Department of Applied Chemistry, Faculty of Chemistry, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain., Cramer T; Department of Physics and Astronomy, University of Bologna, Viale Carlo Berti Pichat 6/2, 40127 Bologna, Italy., Antognazza MR; Center for Nano Science and Technology@PoliMi, Istituto Italiano di Tecnologia, Via Raffaele Rubattino 81, 20134 Milano, Italy., Mecerreyes D; POLYMAT, University of the Basque Country UPV/EHU, Paseo Manuel de Lardizabal 3, 20018 Donostia-San Sebastián, Spain.; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain.
Jazyk: angličtina
Zdroj: Nano letters [Nano Lett] 2024 Jun 06. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1021/acs.nanolett.4c01195
Abstrakt: The integration of nanotechnology with photoredox medicine has led to the emergence of biocompatible semiconducting polymer nanoparticles (SPNs) for the optical modulation of intracellular reactive oxygen species (ROS). However, the need for efficient photoactive materials capable of finely controlling the intracellular redox status with high spatial resolution at a nontoxic light density is still largely unmet. Herein, highly photoelectrochemically efficient photoactive polymer beads are developed. The photoactive material/electrolyte interfacial area is maximized by designing porous semiconducting polymer nanoparticles (PSPNs). PSPNs are synthesized by selective hydrolysis of the polyester segments of nanoparticles made of poly(3-hexylthiophene)- graft -poly(lactic acid) (P3HT- g -PLA). The photocurrent of PSPNs is 4.5-fold higher than that of nonporous P3HT- g -PLA-SPNs, and PSPNs efficiently reduce oxygen in an aqueous environment. PSPNs are internalized within endothelial cells and optically trigger ROS generation with a >1.3-fold concentration increase with regard to nonporous P3HT-SPNs, at a light density as low as a few milliwatts per square centimeter, fully compatible with in vivo , chronic applications.
Databáze: MEDLINE
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