[Determination of six bisphenols in human urine by ultra-high performance liquid chromatography-tandem mass spectrometry].

Autor:	Pei Z; NHC Key Laboratory of Public Nutrition and Health, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Zhuo Q; NHC Key Laboratory of Public Nutrition and Health, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Zhang L; China National Center for Food Safety Risk Assessment, Chinese Academy of Medical Sciences Research Unit ( No.2019RU014), Beijing 100021, China., Li J; China National Center for Food Safety Risk Assessment, Chinese Academy of Medical Sciences Research Unit ( No.2019RU014), Beijing 100021, China., Zhao Y; China National Center for Food Safety Risk Assessment, Chinese Academy of Medical Sciences Research Unit ( No.2019RU014), Beijing 100021, China.
Jazyk:	čínština
Zdroj:	Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2024 May; Vol. 53 (3), pp. 447-454.
DOI:	10.19813/j.cnki.weishengyanjiu.2024.03.016
Abstrakt:	Objective: To develop and validate a solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry method for the determination of six bisphenols(bisphenol S, bisphenol F, bisphenol A, 2, 2'-methylenediphenol, bisphenol AF, bisphenol AP) in urine. Methods: After enzymolysis of urine sample, the target substances were quickly purified and extracted by WAX solid phase extraction column. On ACQUITY BEH C_(18) column(2.1 mm×100 mm, 1.7 μm), the mobile phase of water and methanol was used to separate. Finally, multi-reaction detection was carried out under electrospray negative ion scanning, and quantification was carried out by internal standard method. Results: The correlation coefficients(r) of the target compounds were all more than 0.998 in the range of 0.1-50.0 ng/mL, the linearity was good, and the detection limits were all lower than 0.1 ng/mL. The recoveries of the three standard concentrations(0.5, 5.0 and 50.0 ng/mL) were all between 80% and 120%, and the relative standard deviation was less than 20%(n=5). The standard reference material was detected and the concentration was within the reference range. Conclusion: This method can be used to detect six bisphenols in urine quickly and accurately, is suitable for the trace analysis of bisphenol compounds in human urine.
Databáze:	MEDLINE
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