Study on the interaction between agglutinin and chondroitin sulfate and dermatan sulfate using multiple methods.

Autor: Liu S; School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China., Zhang X; Shandong Lukang Pharmaceutical Co., LTD, Jining 272000, China., Chen Y; School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China., Li Y; School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China., Liu X; School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China. Electronic address: liuxium@sdu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 272 (Pt 1), pp. 132624. Date of Electronic Publication: 2024 Jun 04.
DOI: 10.1016/j.ijbiomac.2024.132624
Abstrakt: In this work, the interaction of chondroitin sulfate (CS) and dermatan sulfate (DS) with plant lectins was studied by affinity capillary electrophoresis (ACE), surface plasmon resonance (SPR) technology, molecular docking simulation, and circular dichroism spectroscopy. The ACE method was used for the first time to study the interaction of Ricinus Communis Agglutinin I (RCA I), Wisteria Floribunda Lectin (WFA), and Soybean Agglutinin (SBA) with CS and DS, and the results were in good agreement with those of the SPR method. The results of experiments indicate that RCA I has a strong binding affinity with CS, and the sulfated position does not affect the relationship, but the degree of sulfation can affect the combination of RCA I with CS to some extent. However, the binding affinity with DS is very weak. This study lays the foundation for developing more specialized analysis methods for CS and DS based on RCA I.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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