Conversion obstacle from Mg-protoporphyrin IX to protochlorophyllide might be responsible for chlorophyll-deficient phenotype of the Huangjinya's albino offspring.

Autor: Li CY; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: Cunyu.li@zju.edu.cn., Hu SY; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: 125818605@qq.com., Yang WT; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China; Shanghai East Pudong Foreign Language School, Shanghai, 201203, PR China. Electronic address: 2694885455@qq.com., Yang HZ; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: 22216225@zju.edu.cn., Zhang WW; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: 12116072@zju.edu.cn., Ye JH; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: jx0515@163.com., Zheng XQ; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: xqzheng@zju.edu.cn., Liang YR; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: yrliang@zju.edu.cn., Dong ZB; Agricultural and Rural Bureau of Pingyang County, Wenzhou, 325405, PR China. Electronic address: dozhanbo@gmail.com., Lu JL; Tea Research Institute, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: jllu@zju.edu.cn.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Jul; Vol. 212, pp. 108778. Date of Electronic Publication: 2024 May 31.
DOI: 10.1016/j.plaphy.2024.108778
Abstrakt: The albino tea cultivar is one of the most important germplasms for key gene mining and high-quality tea producing. In order to elucidate the chlorophyll-deficient mechanism of albino cultivar 'Huangjinya' and its offspring, color difference, photosynthetic pigments and the relevant genes' expression of the tender shoots were comprehensively investigated in this study. Among the tested 16 offspring, 5 exhibited albino phenotype in spring and autumn, 3 showed albino phenotype in spring but normal green in autumn, while the rests were all normal green. The shoot of albino offspring had significantly higher lightness and/or yellowness than that of green ones, and possessed dramatically lower photosynthetic pigments and chlorophyll precursor protochlorophyllide (Pchlide), as well as higher chlorophyll a/chlorophyll b but lower chlorophylls/carotenoids in comparison with green ones. Among the tested genes involved in chlorophyll and carotenoid metabolism pathways, expression of the magnesium protoporphyrin IX monomethyl ester cyclase (CRD), 3,8-divinyl chlorophyllide 8-vinyl reductase (DVR), 5-aminolevulinate dehydratase 1 (HEMB1), 1-deoxy-D-xylulose 5-phosphate synthase 1 (DXS1) and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ISPH) was remarkably down-regulated in shoots of the albino offspring. Color difference indices of the offspring were significantly correlated with the levels of photosynthetic pigments and Pchlide, and low level of chlorophylls in shoot of albino offspring was mainly due to conversion obstacle from magnesium protoporphyrin Ⅸ (Mg-Proto IX) to Pchlide which might be attributed to down-regulatory expression of CRD and DVR.
Competing Interests: Declaration of competing interest The authors declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: