Ultrastructural changes, pigment responses and bioaccumulation in the microalga Phaeodactylum tricornutum Bohlin exposed to BPA analogues.

Autor: Fabrello J; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy. Electronic address: jacopofabrello@gmail.com., Guidorizzi S; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Ciscato M; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Battistuzzi M; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Moschin E; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Dalla Vecchia F; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Moro I; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy., Roverso M; Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy., Bogialli S; Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy., Matozzo V; Department of Biology, University of Padova, Via Basssi 58/B, 35131 Padova, Italy.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2024 Jul; Vol. 272, pp. 106970. Date of Electronic Publication: 2024 May 21.
DOI: 10.1016/j.aquatox.2024.106970
Abstrakt: As well-known, microalgae have a pivotal role in aquatic environments, being the primary producer. In this study, we investigated the effects of Bisphenol A (BPA) analogues on cell ultrastructure, reactive oxygen species (ROS) production and photosynthetic pigment responses in the diatom Phaeodactylum tricornutum. Microalgae were exposed during both exponential and stationary growth phases to an environmental relevant concentration (300 ng/L) of three differing BPA analogues (BPAF, BPF, and BPS) and their mixture (100 ng/L of each compound). Bioaccumulation of such compounds in microalgae was also analysed. During the stationary growth phase, a significant increase in the percentage of cells with hydrogen peroxide production was recorded after exposure to both BPS and MIX. Conversely, no significant effects on total chlorophylls and carotenoids were observed. During exponential growth phase we observed that control cultures had chloroplasts with well-organized thylakoid membranes and a central pyrenoid. On the contrary, the culture cells treated with BPA analogues and MIX showed chloroplasts characterized by evident dilation of thylakoid membranes. The presence of degeneration areas in the cytoplasm was also recorded. During the stationary growth phase, control and culture cells were characterized by chloroplasts with a regular thylakoid system, whereas BPA analogues-exposed cells were characterized by a deep degradation of the cytoplasm but showed chloroplasts without evident alterations of the thylakoid system. Lipid bodies were visible in treated microalgae. Lastly, microalgae bioaccumulated mainly BPS and BPF, alone or in the MIX. Overall, results obtained revealed that BPA analogues can affect some important biochemical and ultrastructure features of microalgae, promoting ROS production. Lastly, the capability of microalgae to bioaccumulate bisphenols suggest a potential ecotoxicological risk for filter-feeders organisms.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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