Enhancing guided bone regeneration with cross-linked collagen-conjugated xenogeneic bone blocks and membrane fixation: A preclinical in vivo study.

Autor: An YZ; Department of Periodontology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou, Guangdong, China., Song YW; Department of Periodontology, Dental Hospital, Veterans Health Service Medical Center, Seoul, Korea., Thoma DS; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.; Clinic of Reconstructive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland., Strauss FJ; Clinic of Reconstructive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.; Faculty of Dentistry, Universidad Finis Terrae, Santiago, Chile., Lee JS; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.; Innovation Research and Support Center for Dental Science, Yonsei University Dental Hospital, Seoul, Korea.
Jazyk: angličtina
Zdroj: Clinical oral implants research [Clin Oral Implants Res] 2024 Oct; Vol. 35 (10), pp. 1226-1239. Date of Electronic Publication: 2024 Jun 05.
DOI: 10.1111/clr.14309
Abstrakt: Objective: To determine whether combining cross-linked (CL) collagen-integrated xenogeneic bone blocks stabilized with the fixation of resorbable collagen membranes (CM) can enhance guided bone regeneration (GBR) in the overaugmented calvarial defect model.
Materials and Methods: Four circular defects with a diameter of 8 mm were prepared in the calvarium of 13 rabbits. Defects were randomly assigned to receive one of the following treatments: (i) non-cross-linked (NCL) porcine-derived collagen-embedded bone block covered by a CM without fixation (NCL + unfix group); (ii) NCL bone block covered by CM with fixation using bone-tack (NCL + fix group); (iii) cross-linked (CL) porcine-derived collagen-embedded bone block covered by CM without fixation (CL + unfix group); and (iv) CL bone block covered by CM with fixation using bone-tack fixation (CL + fix group). The efficacy of GBR was assessed through histological and molecular analyses after 2 and 8 weeks.
Results: At 2 weeks, there were no significant differences in histologically measured areas of newly formed bone among the groups. At 8 weeks, however, the CL + fix group exhibited a larger area of new bone (5.08 ± 1.09 mm 2 , mean ± standard deviation) compared to the NCL + unfix (1.62 ± 0.42 mm 2 ; p < .0083), NCL + fix (3.97 ± 1.39 mm 2 ) and CL + unfix (2.55 ± 1.04 mm 2 ) groups. Additionally, the expression levels of tumour necrosis factor-alpha, fibroblast growth factor-2, vascular endothelial growth factor, osteocalcin and calcitonin receptor were significantly higher in the CL + fix group compared to the other three groups (p < .0083).
Conclusion: Cross-linked bone blocks stabilized with collagen membrane fixation can significantly enhance GBR.
(© 2024 The Author(s). Clinical Oral Implants Research published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: