The molecular architecture of Lactobacillus S-layer: Assembly and attachment to teichoic acids.

Autor:	Sagmeister T; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Gubensäk N; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Buhlheller C; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Grininger C; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Eder M; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Ðordić A; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Millán C; Structural Biology Unit, Institute of Molecular Biology of Barcelona, Spanish National Research Council, Barcelona 08028, Spain., Medina A; Structural Biology Unit, Institute of Molecular Biology of Barcelona, Spanish National Research Council, Barcelona 08028, Spain., Murcia PAS; Laboratory of Computer-Aided Molecular Design, Division of Medicinal Chemistry, Otto-Loewi Research Center, Medical University of Graz, Graz, Austria 8010., Berni F; Department of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden 2333, The Netherlands., Hynönen U; Department of Basic Veterinary Sciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki 00100, Finland., Vejzović D; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Damisch E; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Kulminskaya N; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Petrowitsch L; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010., Oberer M; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010.; Field of Excellence BioHealth, University of Graz, Graz 8010, Austria.; BioTechMed-Graz, University of Graz, Graz 8010, Austria., Palva A; Department of Basic Veterinary Sciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki 00100, Finland., Malanović N; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010.; Field of Excellence BioHealth, University of Graz, Graz 8010, Austria.; BioTechMed-Graz, University of Graz, Graz 8010, Austria., Codée J; Department of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden 2333, The Netherlands., Keller W; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010.; Field of Excellence BioHealth, University of Graz, Graz 8010, Austria.; BioTechMed-Graz, University of Graz, Graz 8010, Austria., Usón I; Structural Biology Unit, Institute of Molecular Biology of Barcelona, Spanish National Research Council, Barcelona 08028, Spain.; Institució Catalana de Recerca i Estudis Avançats, Barcelona 08003, Spain., Pavkov-Keller T; Institute of Molecular Biosciences, University of Graz, Graz, Austria 8010.; Field of Excellence BioHealth, University of Graz, Graz 8010, Austria.; BioTechMed-Graz, University of Graz, Graz 8010, Austria.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 11; Vol. 121 (24), pp. e2401686121. Date of Electronic Publication: 2024 Jun 05.
DOI:	10.1073/pnas.2401686121
Abstrakt:	S-layers are crystalline arrays found on bacterial and archaeal cells. Lactobacillus is a diverse family of bacteria known especially for potential gut health benefits. This study focuses on the S-layer proteins from Lactobacillus acidophilus and Lactobacillus amylovorus common in the mammalian gut. Atomic resolution structures of Lactobacillus S-layer proteins SlpA and SlpX exhibit domain swapping, and the obtained assembly model of the main S-layer protein SlpA aligns well with prior electron microscopy and mutagenesis data. The S-layer's pore size suggests a protective role, with charged areas aiding adhesion. A highly similar domain organization and interaction network are observed across the Lactobacillus genus. Interaction studies revealed conserved binding areas specific for attachment to teichoic acids. The structure of the SlpA S-layer and the suggested incorporation of SlpX as well as its interaction with teichoic acids lay the foundation for deciphering its role in immune responses and for developing effective treatments for a variety of infectious and bacteria-mediated inflammation processes, opening opportunities for targeted engineering of the S-layer or lactobacilli bacteria in general. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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