IFN-γ stimulates Paneth cell secretion through necroptosis mTORC1 dependent.

Autor: Encarnacion-Garcia MR; Departament of Physiology, Biophysics, and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., De la Torre-Baez R; Departament of Physiology, Biophysics, and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Hernandez-Cueto MA; Clinical Laboratory of Infectology, National Hospital 'La Raza' Medical Center, IMSS, Mexico City, Mexico., Velázquez-Villegas LA; Physiology of Nutrition Department, The National Institute of Health Sciences and Nutrition 'Salvador Zubirán', Mexico City, Mexico., Candelario-Martinez A; Departament of Physiology, Biophysics, and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Sánchez-Argáez AB; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Horta-López PH; Departament of Physiology, Biophysics, and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Montoya-García A; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Jaimes-Ortega GA; Immunology and Proteomics Research Laboratory, Children's Hospital of Mexico 'Federico Gómez' (HIMFG), Mexico City, Mexico.; Experimental Biology Postgraduate Program, Department of Biological and Health Sciences, Metropolitan Autonomous University (UAM), Mexico City, Mexico., Lopez-Bailon L; Immunology Department and Immunology Postgraduate Program, National School of Biological Sciences of the National Polytechnic Institute (ENCB-IPN), Mexico City, Mexico., Piedra-Quintero Z; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Carrasco-Torres G; CICATA Unidad Morelos, Instituto Politécnico Nacional, Boulevard de la Tecnología, 1036 Z-1, P 2/2, Atlacholoaya, 62790, México., De Ita M; Department of Genetics and Molecular Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico.; Medical Research Unit in Human Genetics, UMAE Children's Hospital, National Medical Center 'Siglo XXI', IMSS, Ciudad de México, 06720, Mexico., Figueroa-Corona MDP; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Muñoz-Medina JE; Clinical Laboratory of Infectology, National Hospital 'La Raza' Medical Center, IMSS, Mexico City, Mexico., Sánchez-Uribe M; Pathological Anatomy, Specialized hospital 'Dr. Antonio Fraga Mouret', National Hospital 'La Raza' Medical Center, IMSS, Ciudad de México, México., Ortiz-Fernández A; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Meraz-Ríos MA; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Silva-Olivares A; Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Betanzos A; Departament of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Baay-Guzman GJ; Unit of Investigative Research on Oncological Diseases, Children's Hospital of Mexico 'Federico Gomez', Mexico City, Mexico., Navarro-Garcia F; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Villa-Treviño S; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Garcia-Sierra F; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Cisneros B; Department of Genetics and Molecular Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Schnoor M; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Ortíz-Navarrete VF; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Villegas-Sepúlveda N; Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico., Valle-Rios R; Immunology and Proteomics Research Laboratory, Children's Hospital of Mexico 'Federico Gómez' (HIMFG), Mexico City, Mexico.; University Research Unit, Research Division, Faculty of Medicine, National Autonomous University of Mexico-Children's Hospital of Mexico 'Federico Gomez' (UNAM-HIMFG), Mexico City, Mexico., Medina-Contreras O; Epidemiology, Endocrinology & Nutrition Research Unit, Children's Hospital of Mexico 'Federico Gomez', Mexico City, Mexico., Noriega LG; Physiology of Nutrition Department, The National Institute of Health Sciences and Nutrition 'Salvador Zubirán', Mexico City, Mexico., Nava P; Departament of Physiology, Biophysics, and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Mexico City, Mexico.
Jazyk: angličtina
Zdroj: European journal of immunology [Eur J Immunol] 2024 Jun 05, pp. e2350716. Date of Electronic Publication: 2024 Jun 05.
DOI: 10.1002/eji.202350716
Abstrakt: Immune mediators affect multiple biological functions of intestinal epithelial cells (IECs) and, like Paneth and Paneth-like cells, play an important role in intestinal epithelial homeostasis. IFN-γ a prototypical proinflammatory cytokine disrupts intestinal epithelial homeostasis. However, the mechanism underlying the process remains unknown. In this study, using in vivo and in vitro models we demonstrate that IFN-γ is spontaneously secreted in the small intestine. Furthermore, we observed that this cytokine stimulates mitochondrial activity, ROS production, and Paneth and Paneth-like cell secretion. Paneth and Paneth-like secretion downstream of IFN-γ, as identified here, is mTORC1 and necroptosis-dependent. Thus, our findings revealed that the pleiotropic function of IFN-γ also includes the regulation of Paneth cell function in the homeostatic gut.
(© 2024 The Author(s). European Journal of Immunology published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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