Pilot study to evaluate isolation by size of circulating tumour cells in canine oral melanoma.

Autor: Nowosh V; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Braun AC; Secretaria de Ciência, Tecnologia e Inovação do Complexo Econômico-Industrial da Saúde, Coordenação Geral de Pesquisa Clínica, Ministério da Saúde, Brasilia, Brazil., Ruano APC; International Research Center, A.C. Camargo Cancer Center, São Paulo, Brazil., Chinén LTD; Associação Beneficente Siria, Hcor e Hospital Amaral Carvalho, Jaú, Brazil., de Oliveira Massoco C; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Veterinary and comparative oncology [Vet Comp Oncol] 2024 Sep; Vol. 22 (3), pp. 388-397. Date of Electronic Publication: 2024 Jun 04.
DOI: 10.1111/vco.12982
Abstrakt: Liquid biopsy for circulating tumour cell (CTC) detection is generally unexplored in veterinary medicine. Dogs with highly aggressive and heterogeneous tumours, such as oral malignant melanoma (OMM), could benefit from studies involving size-based isolation methods for CTCs, as they do not depend on specific antibodies. This pilot study aimed to detect CTCs from canine OMM using Isolation by Size of Epithelial Tumor Cells (ISET), a microfiltration methodology, followed by immunocytochemistry (ICC) with Melan-A, PNL2, and S100 antibodies. Ten canine patients diagnosed by histopathology and confirmed as OMM by immunohistochemistry were enrolled, their prognostic data was assessed, and blood samples were collected for CTC analysis. Results have shown the detection of intact cells in 9/10 patients. ICC has shown 3/9 Melan-A-positive, 3/9 PNL2-positive, and 8/9 S100-positive patients, confirming the importance of opting for a multimarker assay. A significant number of negative-stained CTCs were found, suggesting their high heterogeneity in circulation. Microemboli stained with either PNL2 or S100 were found in a patient with a high isolated cell count and advanced clinical stage. Preliminary statistical analysis shows a significant difference in CTC count between patients with and without lymph node metastasis (p < .05), which may correlate with tumour metastatic potential. However, we recommend further studies with more extensive sampling to confirm this result. This pilot study is the first report of intact CTC detection in canine OMM and the first application of ISET in veterinary medicine, opening new possibilities for liquid biopsy studies in canine OMM and other tumours.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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