In Vitro Cell Surface Marker Expression on Mesenchymal Stem Cell Cultures does not Reflect Their Ex Vivo Phenotype.

Autor: Cao Y; Department of Molecular Medicine and Pathology, University of Auckland, Private Bag 92-019, Auckland, 1142, New Zealand., Boss AL; Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand., Bolam SM; Department of Surgery, University of Auckland, Auckland, New Zealand., Munro JT; Department of Surgery, University of Auckland, Auckland, New Zealand., Crawford H; Starship Childrens Hospital, Auckland, New Zealand., Dalbeth N; Department of Medicine, University of Auckland, Auckland, New Zealand., Poulsen RC; Department of Pharmacology, University of Auckland, Auckland, New Zealand., Matthews BG; Department of Molecular Medicine and Pathology, University of Auckland, Private Bag 92-019, Auckland, 1142, New Zealand. brya.matthews@auckland.ac.nz.
Jazyk: angličtina
Zdroj: Stem cell reviews and reports [Stem Cell Rev Rep] 2024 Aug; Vol. 20 (6), pp. 1656-1666. Date of Electronic Publication: 2024 Jun 05.
DOI: 10.1007/s12015-024-10743-1
Abstrakt: Cell surface marker expression is one of the criteria for defining human mesenchymal stem or stromal cells (MSC) in vitro. However, it is unclear if expression of markers including CD73 and CD90 reflects the in vivo origin of cultured cells. We evaluated expression of 15 putative MSC markers in primary cultured cells from periosteum and cartilage to determine whether expression of these markers reflects either the differentiation state of cultured cells or the self-renewal of in vivo populations. Cultured cells had universal and consistent expression of various putative stem cell markers including > 95% expression CD73, CD90 and PDPN in both periosteal and cartilage cultures. Altering the culture surface with extracellular matrix coatings had minimal effect on cell surface marker expression. Osteogenic differentiation led to loss of CD106 and CD146 expression, however CD73 and CD90 were retained in > 90% of cells. We sorted freshly isolated periosteal populations capable of CFU-F formation on the basis of CD90 expression in combination with CD34, CD73 and CD26. All primary cultures universally expressed CD73 and CD90 and lacked CD34, irrespective of the expression of these markers ex vivo indicating phenotypic convergence in vitro. We conclude that markers including CD73 and CD90 are acquired in vitro in most 'mesenchymal' cells capable of expansion. Overall, we demonstrate that in vitro expression of many cell surface markers in plastic-adherent cultures is unrelated to their expression prior to culture.
(© 2024. The Author(s).)
Databáze: MEDLINE
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