Spray vaccination with a Newcastle disease virus (NDV)-vectored infectious laryngotracheitis (ILT) vaccine protects commercial chickens from ILT in the presence of maternally-derived antibodies.

Autor:	Zeng Z; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Wang Z; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Wang X; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Yao L; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Shang Y; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Feng H; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Wang H; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Shao H; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China., Luo Q; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, People's Republic of China., Wen G; Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, People's Republic of China.; Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, People's Republic of China.; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, People's Republic of China.; Hubei Hongshan Laboratory, Wuhan, People's Republic of China.
Jazyk:	angličtina
Zdroj:	Avian pathology : journal of the W.V.P.A [Avian Pathol] 2024 Dec; Vol. 53 (6), pp. 533-539. Date of Electronic Publication: 2024 Jul 11.
DOI:	10.1080/03079457.2024.2356676
Abstrakt:	Infectious laryngotracheitis (ILT) poses a significant threat to the poultry industry, and vaccines play an important role in protection. However, due to the increasing scale of poultry production, there is an urgent need to develop vaccines that are suitable for convenient immunization methods such as spraying. Previous studies have shown that Newcastle disease virus (NDV)-ILT vaccines administered via intranasal and intraocular routes to commercial chickens carrying maternally-derived antibodies (MDAs) are still protective against ILT. In this study, a recombinant NDV (rNDV) was generated to express infectious laryngotracheitis virus (ILTV) glycoprotein B (gB), named rLS-gB, based on a full-length cDNA clone of the LaSota strain. The protective effect of different doses of rLS-gB administered by spray vaccination to commercial chickens at 1 d of age (doa) was evaluated. The chickens were exposed to 160-μm aerosol particles for 10 min for spray vaccination, and no adverse reactions were observed after vaccination. Despite the presence of anti-NDV MDAs and anti-ILTV MDAs in chickens, the ILTV- and NDV-specific antibody titres were significantly greater in the vaccinated groups than in the unvaccinated group. After challenge with a virulent ILTV strain, no clinical signs were observed in the 10 7 EID 50 /ml group compared to the other groups. Furthermore, vaccination with 10 7 EID 50 /ml rLS-gB significantly reduced the ILTV viral load and ameliorated gross and microscopic lesions in the trachea of chickens. Overall, these results suggested that rLS-gB is a safe and efficient candidate spray vaccine for ILT and is especially suitable for scaled chicken farms.
Databáze:	MEDLINE
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