Evidence for metabolism of creatine by the conceptus, placenta, and uterus for production of adenosine triphosphate during conceptus development in pigs†.

Autor:	Lefevre CM; Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA., Cain JW; Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA., Kramer AC; Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA., Seo H; Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA., Lopez AN; Department of Animal Science, Texas A&M University, College Station, TX, USA., Sah N; Department of Animal Science, Texas A&M University, College Station, TX, USA., Wu G; Department of Animal Science, Texas A&M University, College Station, TX, USA., Bazer FW; Department of Animal Science, Texas A&M University, College Station, TX, USA., Johnson GA; Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2024 Sep 14; Vol. 111 (3), pp. 694-707.
DOI:	10.1093/biolre/ioae088
Abstrakt:	In pigs, the majority of embryonic mortality occurs when free-floating conceptuses (embryos/fetuses and associated placental membranes) elongate, and the uterine-placental interface undergoes folding and develops areolae. Both periods involve proliferation, migration, and changes in morphology of cells that require adenosine triphosphate (ATP). We hypothesize that insufficient ATP in conceptus and uterine tissues contributes to conceptus loss in pigs. Creatine is stored in cells as phosphocreatine for ATP regeneration through the creatine-creatine kinase- phosphocreatine pathway. However, the expression of components of this pathway in pigs has not been examined throughout gestation. Results of qPCR analyses indicated increases in AGAT, GAMT, CKM, CKB, and SLC6A8 mRNAs in elongating porcine conceptuses, and immunofluorescence microscopy localized guanidinoacetate N-methyltransferase, creatine kinase M, and creatine kinase B proteins to the trophectoderm of elongating conceptuses, to the columnar chorionic epithelial cells at the bottom of chorioallantoic troughs, and to endometrial luminal epithelium at the tops of the endometrial ridges of uterine-placental folds on Days 40, 60, and 90 of gestation. Guanidinoacetate N-methyltransferase protein is expressed in endometrial luminal epithelium at the uterine-placental interface, but immunostaining is more intense in luminal epithelium at the bottoms of the endometrial ridges. Results of this study indicate that key elements of the pathway for creatine metabolism are expressed in cells of the conceptus, placenta, and uterus for potential production of ATP during two timepoints in pregnancy with a high demand for energy; elongation of the conceptus for implantation and development of uterine-placental folding during placentation. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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