Optimizing GABA A receptor antagonism for the induction of long-term potentiation in the mouse and rat dentate gyrus in vitro.

Autor:	Eyolfson E; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Acosta C; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Brand J; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Suesser KRB; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Kannangara T; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Bostrom C; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada.; The Island Medical Program and Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada., Sawchuk S; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada., Christie BR; Division of Medical Sciences and Institute for Aging and Lifelong Health, University of Victoria, Victoria, British Columbia, Canada.; The Island Medical Program and Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Psychology, San Diego State University, San Diego, California, United States.
Jazyk:	angličtina
Zdroj:	Journal of neurophysiology [J Neurophysiol] 2024 Jul 01; Vol. 132 (1), pp. 177-183. Date of Electronic Publication: 2024 Jun 05.
DOI:	10.1152/jn.00188.2024
Abstrakt:	The reliable induction of long-term potentiation (LTP) in the dentate gyrus (DG) in vitro requires the blockade of the γ-aminobutyric acid A (GABA A ) receptor. In these studies we examined the effectiveness of the specific GABA A receptor antagonist bicuculline methiodide (BMI) in facilitating LTP in the DG from hippocampal slices obtained from either C57Bl/6 mice or Sprague-Dawley rats, two species commonly used for electrophysiology. In the C57Bl/6 mice, maximal short-term potentiation and LTP in the DG were produced with a concentration of 5 µM BMI. In contrast, a concentration of 10 μM BMI was required to produce maximal short-term potentiation and LTP in the DG of Sprague-Dawley rats. These results reveal that there are species differences in the optimal amount of BMI required to produce robust and reliable LTP in the rodent DG in vitro and highlight the need to take consideration of the species being used when choosing concentrations of pharmacological agents to employ for electrophysiological use. NEW & NOTEWORTHY In this report we provide specific neurophysiological evidence for concentrations of GABA A antagonist required to study long-term potentiation in the medial perforant pathway of the dentate gyrus. Two commonly used species, Sprague-Dawley rats and C57Bl/6 mice, require different concentrations of bicuculline methiodide to induce optimal short-term and long-term potentiation.
Databáze:	MEDLINE
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