Dietary 5-hydroxytryptophan improves sheep growth performance by enhancing ruminal functions, antioxidant capacity, and tryptophan metabolism: in vitro and in vivo studies.

Autor:	Sun Z; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, China.; Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China., Aschalew ND; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; College of Agriculture and Environmental Science, Dilla University, Dilla, Ethiopia., Cheng L; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Xia Y; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Zhang L; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Yin G; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Wang S; College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, China., Wang Z; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Dong J; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Zhang W; Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China., Zhao W; Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China., Qin G; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Zhang X; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China., Zhong R; Jilin Province Feed Processing and Ruminant Precision Breeding Cross-Regional Cooperation Technology Innovation Center, Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China., Wang T; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China., Zhen Y; Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.; Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2024 May 21; Vol. 15, pp. 1398310. Date of Electronic Publication: 2024 May 21 (Print Publication: 2024).
DOI:	10.3389/fimmu.2024.1398310
Abstrakt:	Background: Hydroxytryptophan (5-HTP) can regulate the synthesis of 5-Hydroxytryptamine (5-HT) and melatonin (MT). In a previous metabolome analysis, we found that 5-HTP is an effective ingredient in yeast culture for regulating rumen fermentation. However, research on the effect of this microbial product (5-HTP) as a functional feed additive in sheep production is still not well explained. Therefore, this study examined the effects of 5-HTP on sheep rumen function and growth performance using in vitro and in vivo models. Methods: A two-factor in vitro experiment involving different 5-HTP doses and fermentation times was conducted. Then, in the in vivo experiment, 10 sheep were divided into a control group which was fed a basal diet, and a 5-HTP group supplemented with 8 mg/kg 5-HTP for 60 days. Results: The results showed that 5-HTP supplementation had a significant effect on in vitro DMD, pH, NH 3 -N, acetic acid, propionic acid, and TVFA concentrations. 5-HTP altered rumen bacteria composition and diversity indices including Chao1, Shannon, and Simpson. Moreover, the in vivo study on sheep confirmed that supplementing with 8 mg/kg of 5-HTP improved rumen fermentation efficiency and microbial composition. This led to enhanced sheep growth performance and increased involvement in the tryptophan metabolic pathway, suggesting potential benefits. Conclusion: Dietary 5-HTP (8 mg/kg DM) improves sheep growth performance by enhancing ruminal functions, antioxidant capacity, and tryptophan metabolism. This study can provide a foundation for the development of 5-HTP as a functional feed additive in ruminants' production. Competing Interests: ZS, WZhan, WZhao, TW, and YZ are employed by Changchun Borui Science and Technology Co., Ltd., China. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Sun, Aschalew, Cheng, Xia, Zhang, Yin, Wang, Wang, Dong, Zhang, Zhao, Qin, Zhang, Zhong, Wang and Zhen.)
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