| Autor: |
Saldanha R; Physics Department, Syracuse University , Syracuse, NY, USA.; BioInspired Institute, Syracuse University , Syracuse, NY, USA., Ho Thanh MT; Physics Department, Syracuse University , Syracuse, NY, USA.; BioInspired Institute, Syracuse University , Syracuse, NY, USA., Krishnan N; BioInspired Institute, Syracuse University , Syracuse, NY, USA.; Department of Biology, Syracuse University , Syracuse, NY, USA., Hehnly H; BioInspired Institute, Syracuse University , Syracuse, NY, USA.; Department of Biology, Syracuse University , Syracuse, NY, USA., Patteson A; Physics Department, Syracuse University , Syracuse, NY, USA.; BioInspired Institute, Syracuse University , Syracuse, NY, USA. |
| Abstrakt: |
Cell polarity is important for controlling cell shape, motility and cell division processes. Vimentin intermediate filaments are important for cell migration and cell polarization in mesenchymal cells and assembly of vimentin and microtubule networks is dynamically coordinated, but the precise details of how vimentin mediates cell polarity remain unclear. Here, we characterize the effects of vimentin on the structure and function of the centrosome and the stability of microtubule filaments in wild-type and vimentin-null mouse embryonic fibroblasts. We find that vimentin mediates the structure of the pericentriolar material, promotes centrosome-mediated microtubule regrowth and increases the level of stable acetylated microtubules in the cell. Loss of vimentin also impairs centrosome repositioning during cell polarization and migration processes that occur during wound closure. Our results suggest that vimentin modulates centrosome structure and function as well as microtubule network stability, which has important implications for how cells establish proper cell polarization and persistent migration. |
