Quantifying CO-release from a photo-CORM using 19 F NMR: An investigation into light-induced CO delivery.

Autor: Gandra UR; Institute for Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743, Jena, Germany; Department of Chemistry, Khalifa University of Science and Technology, P.O. Box 127788, Abu Dhabi, United Arab Emirates. Electronic address: upreddygandra@gmail.com., Liu J; Institute for Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743, Jena, Germany., Axthelm J; Institute for Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743, Jena, Germany., Mohamed S; Department of Chemistry, Khalifa University of Science and Technology, P.O. Box 127788, Abu Dhabi, United Arab Emirates; Center for Catalysis and Separations (CeCaS), Khalifa University of Science and Technology, P.O. Box 127788, Abu Dhabi, United Arab Emirates., Görls H; Institute for Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743, Jena, Germany., Mohideen MIH; Department of Chemistry, Khalifa University of Science and Technology, P.O. Box 127788, Abu Dhabi, United Arab Emirates; Center for Catalysis and Separations (CeCaS), Khalifa University of Science and Technology, P.O. Box 127788, Abu Dhabi, United Arab Emirates. Electronic address: mohamed.mohideen@ku.ac.ae., Schiller A; Institute for Inorganic and Analytical Chemistry (IAAC), Friedrich Schiller University Jena, Humboldtstr. 8, D-07743, Jena, Germany. Electronic address: schiller@schillermertens.de.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Jul 11; Vol. 1312, pp. 342749. Date of Electronic Publication: 2024 May 20.
DOI: 10.1016/j.aca.2024.342749
Abstrakt: Carbon monoxide (CO) is an innate signaling molecule that can regulate immune responses and interact with crucial elements of the circadian clock. Moreover, pharmacologically, CO has been substantiated for its therapeutic advantages in animal models of diverse pathological conditions. Given that an excessive level of CO can be toxic, it is imperative to quantify the necessary amount for therapeutic use accurately. However, estimating gaseous CO is notably challenging. Therefore, novel techniques are essential to quantify CO in therapeutic applications and overcome this obstacle precisely. The classical Myoglobin (Mb) assay technique has been extensively used to determine the amount of CO-release from CO-releasing molecules (CORMs) within therapeutic contexts. Nevertheless, specific challenges arise when applying the Mb assay to evaluate CORMs featuring innovative molecular architectures. Here, we report a fluorinated photo-CORM (CORM-FBS) for the photo-induced CO-release. We employed the 19 F NMR spectroscopy approach to monitor the release of CO as well as quantitative evaluation of CO release. This new 19 F NMR approach opens immense opportunities for researchers to develop reliable techniques for identifying molecular structures, quantitative studies of drug metabolism, and monitoring the reaction process.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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