BMSC loaded photo-crosslinked hyaluronic acid/collagen hydrogel incorporating FG4592 for enhanced cell proliferation and nucleus pulposus differentiation.

Autor: Lin CL; Department of Orthopedic Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Su YW; School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Chen YW; School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Kuo CH; International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, Taiwan., Tu TY; Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan., Tsai JC; School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Shyong YJ; School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address: bear901704@gs.ncku.edu.tw.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jul; Vol. 273 (Pt 1), pp. 132828. Date of Electronic Publication: 2024 Jun 02.
DOI: 10.1016/j.ijbiomac.2024.132828
Abstrakt: Intervertebral disc degeneration arises from damage or degeneration of the nucleus pulposus (NP). In this study, we developed a photo-crosslinkable hydrogel incorporating FG4592 to support the growth and differentiation of bone-marrow-derived mesenchymal stem cells (BMSC). Initially, hyaluronic acid was modified with tyramine and combined with collagen to introduce riboflavin as a photo-crosslinker. This hydrogel transitioned from liquid to gel upon exposure to blue light in 3 min. The results showed that the hydrogel was biodegradable and had mechanical properties comparable to those of human NP tissues. Scanning electron microscopy after BMSC seeding in the hydrogel revealed an even distribution, and cells adhered to the collagen fibers in the hydrogel with minimal cell mortality. The effect of FG4592 on BMSC proliferation and differentiation was examined, revealing the capability of FG4592 to promote BMSC proliferation and direct differentiation resembling human NP cells. After cultivating BMSCs in the photo-crosslinked hydrogel, there was an upregulation in the expression of glycosaminoglycans, aggrecan, type II collagen, and keratin 19 proteins. Cross-species analyses of rat and human BMSCs revealed consistent results. For potential clinical applications, BMSC loaded with photo-crosslinked hydrogels can be injected into damaged intervertebral disc to facilitate NP regeneration.
Competing Interests: Declaration of competing interest The authors declare the absence of any competing financial interests or personal relationships that could potentially be perceived as influencing the research presented in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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