| Autor: |
Abdellatef SA; Mechanobiology group, Research Centre for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, Japan. nims.email.Shimaa@gmail.com., Bard F; Mechanobiology group, Research Centre for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, Japan. nims.email.Shimaa@gmail.com.; Department of Material Science and Engineering, Cornell University, Ithaca, NY, USA., Nakanishi J; Mechanobiology group, Research Centre for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, Japan. nims.email.Shimaa@gmail.com.; Waseda University Graduate School of Advanced Science and Engineering Department of Nanoscience and Engineering, Tokyo, Japan.; Tokyo University of Science, advanced Graduate School of Engineering Materials Innovation Engineering, Japan. |
| Abstrakt: |
In cancer metastasis, collectively migrating clusters are discriminated into leader and follower cells that move through extracellular matrices (ECMs) with different characteristics. The impact of changes in ECM protein types on leader cells and migrating clusters is unknown. To address this, we investigated the response of leader cells and migrating clusters upon moving from one ECM protein to another using a photoactivatable substrate bearing photocleavable PEG (PCP), whose surface changes from protein-repellent to protein-adhesive in response to light. We chose laminin and collagen I for our study since they are abundant in two distinct regions in living tissues, namely basement membrane and connective tissue. Using the photoactivatable substrates, the precise deposition of the first ECM protein in the irradiated areas was achieved, followed by creating well-defined cellular confinements. Secondary irradiation enabled the deposition of the second ECM protein in the new irradiated regions, resulting in region-selective heterogeneous and homogenous ECM protein-coated surfaces. Different tendencies in leader cell formation from laminin into laminin compared to those migrating from laminin into collagen were observed. The formation of focal adhesion and actin structures for cells within the same cluster in the ECM proteins responded according to the underlying ECM protein type. Finally, integrin β1 was crucial for the appearance of leader cells for clusters migrating from laminin into collagen. However, when it came to laminin into laminin, integrin β1 was not responsible. This highlights the correlation between leader cells in collective migration and the biochemical signals that arise from underlying extracellular matrix proteins. |
