Scaling behaviour and control of nuclear wrinkling.

Autor: Jackson JA; Department of Biology, Massachusetts Institute of Technology.; Graduate Program in Biophysics, Harvard University., Romeo N; Department of Mathematics, Massachusetts Institute of Technology.; Department of Physics, Massachusetts Institute of Technology., Mietke A; Department of Mathematics, Massachusetts Institute of Technology.; School of Mathematics, University of Bristol., Burns KJ; Department of Mathematics, Massachusetts Institute of Technology., Totz JF; Department of Mathematics, Massachusetts Institute of Technology., Martin AC; Department of Biology, Massachusetts Institute of Technology., Dunkel J; Department of Mathematics, Massachusetts Institute of Technology., Alsous JI; Center for Computational Biology, Flatiron Institute, Simons Foundation.
Jazyk: angličtina
Zdroj: Nature physics [Nat Phys] 2023 Dec; Vol. 19 (12), pp. 1927-1935. Date of Electronic Publication: 2023 Sep 18.
Abstrakt: The cell nucleus is enveloped by a complex membrane, whose wrinkling has been implicated in disease and cellular aging. The biophysical dynamics and spectral evolution of nuclear wrinkling during multicellular development remain poorly understood due to a lack of direct quantitative measurements. Here, we characterize the onset and dynamics of nuclear wrinkling during egg development in the fruit fly when nurse cell nuclei increase in size and display stereotypical wrinkling behavior. A spectral analysis of three-dimensional high-resolution live imaging data from several hundred nuclei reveals a robust asymptotic power-law scaling of angular fluctuations consistent with renormalization and scaling predictions from a nonlinear elastic shell model. We further demonstrate that nuclear wrinkling can be reversed through osmotic shock and suppressed by microtubule disruption, providing tuneable physical and biological control parameters for probing mechanical properties of the nuclear envelope. Our findings advance the biophysical understanding of nuclear membrane fluctuations during early multicellular development.
Databáze: MEDLINE
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