An atlas and database of neuropeptide gene expression in the adult zebrafish forebrain.

Autor: Hiraki-Kajiyama T; Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science, Wako, Saitama, Japan.; Laboratory of Molecular Ethology, Graduate School of Life Science, Tohoku University, Sendai, Miyagi, Japan., Miyasaka N; Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science, Wako, Saitama, Japan., Ando R; Support Unit for Bio-Material Analysis, Research Resources Division, RIKEN Center for Brain Science, Wako, Saitama, Japan., Wakisaka N; Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science, Wako, Saitama, Japan., Itoga H; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan., Onami S; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan.; Life Science Data Sharing Unit, RIKEN Information R&D and Strategy Headquarters, Kobe, Hyogo, Japan., Yoshihara Y; Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science, Wako, Saitama, Japan.
Jazyk: angličtina
Zdroj: The Journal of comparative neurology [J Comp Neurol] 2024 Jun; Vol. 532 (6), pp. e25619.
DOI: 10.1002/cne.25619
Abstrakt: Zebrafish is a useful model organism in neuroscience; however, its gene expression atlas in the adult brain is not well developed. In the present study, we examined the expression of 38 neuropeptides, comparing with GABAergic and glutamatergic neuron marker genes in the adult zebrafish brain by comprehensive in situ hybridization. The results are summarized as an expression atlas in 19 coronal planes of the forebrain. Furthermore, the scanned data of all brain sections were made publicly available in the Adult Zebrafish Brain Gene Expression Database (https://ssbd.riken.jp/azebex/). Based on these data, we performed detailed comparative neuroanatomical analyses of the hypothalamus and found that several regions previously described as one nucleus in the reference zebrafish brain atlas contain two or more subregions with significantly different neuropeptide/neurotransmitter expression profiles. Subsequently, we compared the expression data in zebrafish telencephalon and hypothalamus obtained in this study with those in mice, by performing a cluster analysis. As a result, several nuclei in zebrafish and mice were clustered in close vicinity. The present expression atlas, database, and anatomical findings will contribute to future neuroscience research using zebrafish.
(© 2024 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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