Episodic ozone exposure in Long-Evans rats has limited effects on cauda sperm motility and non-coding RNA populations.

Autor: Chorley BN; Center for Computational Toxicology and Exposure, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Klinefelter GR; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Nelson GM; Center for Computational Toxicology and Exposure, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Strader LF; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Nguyen HH; Oak Ridge Institute for Science and Education, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Schladweiler MC; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Palmer G; Oak Ridge Institute for Science and Education, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Moore ML; Oak Ridge Institute for Science and Education, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Grindstaff RD; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Padgett WT; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Carswell GK; Center for Computational Toxicology and Exposure, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Fisher AA; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Kodavanti UP; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Dye JA; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States., Miller CN; Public Health and Integrated Toxicology Division, Center for Public Health and Environmental Assessment, U S. Environmental Protection Agency, Research Triangle Park, NC, United States. Electronic address: miller.colette@epa.gov.
Jazyk: angličtina
Zdroj: Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2024 Sep; Vol. 128, pp. 108631. Date of Electronic Publication: 2024 Jun 01.
DOI: 10.1016/j.reprotox.2024.108631
Abstrakt: Epidemiological evidence suggests the potential for air pollutants to induce male reproductive toxicity. In experimental studies, exposure to ozone during sensitive windows in the sperm lifecycle has been associated with impaired sperm motility. Subsequently, we sought to investigate the effects of episodic exposure to ozone during sperm maturation in the rat. Long-Evans rats were exposed to either filtered air or ozone (0.4 or 0.8 ppm) for five non-consecutive days over two weeks. Ozone exposure did not impact male reproductive organ weights or sperm motility ∼24 hours following the final exposure. Furthermore, circulating sex hormones remained unchanged despite increased T 3 and T 4 in the 0.8 ppm group. While there was indication of altered adrenergic signaling attributable to ozone exposure in the testis, there were minimal impacts on small non-coding RNAs detected in cauda sperm. Only two piwi-interacting RNAs (piRNAs) were altered in the mature sperm of ozone-exposed rats (piR-rno-346434 and piR-rno-227431). Data across all rats were next analyzed to identify any non-coding RNAs that may be correlated with reduced sperm motility. A total of 7 microRNAs (miRNAs), 8 RNA fragments, and 1682 piRNAs correlated well with sperm motility. Utilizing our exposure paradigm herein, we were unable to substantiate the relationship between ozone exposure during maturation with sperm motility. However, these approaches served to identify a suite of non-coding RNAs that were associated with sperm motility in rats. With additional investigation, these RNAs may prove to have functional roles in the acquisition of motility or be unique biomarkers for male reproductive toxicity.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Corresponding author is co-editor for special issue on reproductive toxicology and air pollution and current sits on the editorial board - CM If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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