Highly fluorescent hybrid nanofibers as potential nanofibrous scaffolds for studying cell-fiber interactions.

Autor: Raja S; National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil; Dept. of Cell and Tumor Biology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse, 30, D-52074 Aachen, Germany; Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, Marcina Strzody Street 9, Gliwice 44-100, Poland; Centre for Organic and Nanohybrid Electronics, Silesian University of Technology, Konarskiego Street 22b, Gliwice 44-100, Poland. Electronic address: Raja.Sebastian@polsl.pl., Paschoalin RT; National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil., Terra IAA; National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil., Schalla C; Dept. of Cell and Tumor Biology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse, 30, D-52074 Aachen, Germany., Guimarães F; São Carlos Institute of Physics, University of São Paulo, Av. Trabalhador São-Carlense, 400, 13566-590 São Carlos, São Paulo, Brazil., Periyasami G; Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia., Mattoso LHC; National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil., Sechi A; Dept. of Cell and Tumor Biology, Medical Faculty, RWTH Aachen University, Pauwelsstrasse, 30, D-52074 Aachen, Germany.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Oct 15; Vol. 319, pp. 124535. Date of Electronic Publication: 2024 May 25.
DOI: 10.1016/j.saa.2024.124535
Abstrakt: In this study, we report on the fabrication of hybrid nanofibers for labeling and bioimaging applications. Our approach is involved for developing highly fluorescent nanofibers using a blend of polylactic acid, polyethyleneglycol, and perylenediimide dyes, through the solution blow spinning technique. The nanofibers are exhibited diameters ranging from 330 nm to 420 nm. Nanofibers showed excellent red and near-infrared fluorescence emissive properties in fluorescent spectroscopy. Moreover, the strong two-photon absorption phenomenon was observed for nanofibers under confocal microscopy. To assess the applicability of these fluorescent nanofibers in bioimaging settings, we employ two types of mammalian cells B16F1 melanoma cells and J774.A1 macrophages. Both cell types exhibit negligible cytotoxicity after 24 h incubation with the nanofibers, indicating the suitability of nanofibers for cell-based experiments. We also observe strong interactions between the nanofibers and cells, as evidenced by two major events: a) the acquisition of an elongated cellular morphology with the major cellular axis parallel to the nanofibers and b) the accumulation of actin filaments along the points of contact of the cells with the fibers. Our findings demonstrate the suitability of these newly developed fluorescent nanofibers in cell-based applications for guiding cellular behavior. We expect that these fluorescent nanofibers have the potential to serve as scaffold materials for long-time tracking of cell-fiber interactions in fluorescence microscopy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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