Evaluating Plasmodium falciparum automatic detection and parasitemia estimation: A comparative study on thin blood smear images.

Autor: Acherar A; Inserm, Institut Pierre-Louis d'Épidémiologie et de Santé Publique, IPLESP, Sorbonne Université, Paris, France.; SCAI (Sorbonne Center for Artificial Intelligence), Sorbonne Université, Paris, France., Tannier X; Sorbonne Université, Inserm, Laboratoire d'Informatique Médicale et d'Ingénierie des Connaissances pour la e-Santé, LIMICS, Université Sorbonne Paris Nord, Paris, France., Tantaoui I; AP-HP, Service de Parasitologie-Mycologie, CNR du Paludisme, Groupe Hospitalier Pitié-Salpêtrière, Paris, France., Brossas JY; AP-HP, Service de Parasitologie-Mycologie, CNR du Paludisme, Groupe Hospitalier Pitié-Salpêtrière, Paris, France., Thellier M; Inserm, Institut Pierre-Louis d'Épidémiologie et de Santé Publique, IPLESP, Sorbonne Université, Paris, France.; AP-HP, Service de Parasitologie-Mycologie, CNR du Paludisme, Groupe Hospitalier Pitié-Salpêtrière, Paris, France., Piarroux R; Inserm, Institut Pierre-Louis d'Épidémiologie et de Santé Publique, IPLESP, Sorbonne Université, Paris, France.; AP-HP, Service de Parasitologie-Mycologie, CNR du Paludisme, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jun 03; Vol. 19 (6), pp. e0304789. Date of Electronic Publication: 2024 Jun 03 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0304789
Abstrakt: Malaria is a deadly disease that is transmitted through mosquito bites. Microscopists use a microscope to examine thin blood smears at high magnification (1000x) to identify parasites in red blood cells (RBCs). Estimating parasitemia is essential in determining the severity of the Plasmodium falciparum infection and guiding treatment. However, this process is time-consuming, labor-intensive, and subject to variation, which can directly affect patient outcomes. In this retrospective study, we compared three methods for measuring parasitemia from a collection of anonymized thin blood smears of patients with Plasmodium falciparum obtained from the Clinical Department of Parasitology-Mycology, National Reference Center (NRC) for Malaria in Paris, France. We first analyzed the impact of the number of field images on parasitemia count using our framework, MALARIS, which features a top-classifier convolutional neural network (CNN). Additionally, we studied the variation between different microscopists using two manual techniques to demonstrate the need for a reliable and reproducible automated system. Finally, we included thin blood smear images from an additional 102 patients to compare the performance and correlation of our system with manual microscopy and flow cytometry. Our results showed strong correlations between the three methods, with a coefficient of determination between 0.87 and 0.92.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Acherar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje