Principles of amyloplast replication in the ovule integuments of Arabidopsis thaliana.

Autor:	Fujiwara MT; Nishina Center and Plant Functions Laboratory (Disbanded in March 2004), RIKEN, Wako, Saitama 351-0198, Japan.; Department of Biology, Graduate School of Science and Technology, Sophia University, Kioicho, Chiyoda 102-8554, Japan.; College of Arts and Sciences, University of Tokyo, Komaba, Tokyo 153-8902, Japan., Yoshioka Y; Graduate School of Science, Nagoya University, Furo-cho, Nagoya 464-8602, Japan., Kazama Y; Nishina Center and Plant Functions Laboratory (Disbanded in March 2004), RIKEN, Wako, Saitama 351-0198, Japan., Hirano T; Nishina Center and Plant Functions Laboratory (Disbanded in March 2004), RIKEN, Wako, Saitama 351-0198, Japan., Niwa Y; Laboratory of Plant Cell Technology, University of Shizuoka, Yada, Shizuoka 422-8526, Japan., Moriyama T; College of Arts and Sciences, University of Tokyo, Komaba, Tokyo 153-8902, Japan., Sato N; College of Arts and Sciences, University of Tokyo, Komaba, Tokyo 153-8902, Japan., Abe T; Nishina Center and Plant Functions Laboratory (Disbanded in March 2004), RIKEN, Wako, Saitama 351-0198, Japan., Yoshida S; Nishina Center and Plant Functions Laboratory (Disbanded in March 2004), RIKEN, Wako, Saitama 351-0198, Japan., Itoh RD; Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Sep 02; Vol. 196 (1), pp. 137-152.
DOI:	10.1093/plphys/kiae314
Abstrakt:	Plastids in vascular plants have various differentiated forms, among which amyloplasts are crucial for starch storage and plant productivity. Despite the vast knowledge of the binary-fission mode of chloroplast division, our understanding of the replication of non-photosynthetic plastids, including amyloplasts, remains limited. Recent studies have suggested the involvement of stromules (stroma-filled tubules) in plastid replication when the division apparatus is faulty. However, details of the underlying mechanism(s) and their relevance to normal processes have yet to be elucidated. Here, we developed a live analysis system for studying amyloplast replication using Arabidopsis (Arabidopsis thaliana) ovule integuments. We showed the full sequence of amyloplast development and demonstrated that wild-type amyloplasts adopt three modes of replication, binary fission, multiple fission, and stromule-mediated fission, via multi-way placement of the FtsZ ring. The minE mutant, with severely inhibited chloroplast division, showed marked heterogeneity in amyloplast size, caused by size-dependent but wild-type modes of plastid fission. The dynamic properties of stromules distinguish the wild-type and minE phenotypes. In minE cells, extended stromules from giant amyloplasts acquired stability, allowing FtsZ ring assembly and constriction, as well as the growth of starch grains therein. Despite hyper-stromule formation, amyloplasts did not proliferate in the ftsZ null mutant. These data clarify the differences between amyloplast and chloroplast replication and demonstrate that the structural plasticity of amyloplasts underlies the multiplicity of their replication processes. Furthermore, this study shows that stromules can generate daughter plastids via the assembly of the FtsZ ring. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu