Viral and host network analysis of the human cytomegalovirus transcriptome in latency.

Autor:	Collins-McMillen D; BIO5 Institute, University of Arizona, Tucson, Arizona, United States of America.; Department of Immunobiology, University of Arizona, Tucson, Arizona, United States of America., De Oliveira Pessoa D; Bioinformatics Shared Resource, Arizona Cancer Center, University of Arizona, Tucson, Arizona, United States of America., Zarrella K; Department of Immunobiology, University of Arizona, Tucson, Arizona, United States of America., Parkins CJ; Vaccine and Gene Therapy Institute, Oregon Health Science University, Beaverton, Oregon, United States of America., Daily M; Vaccine and Gene Therapy Institute, Oregon Health Science University, Beaverton, Oregon, United States of America., Moorman NJ; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Kamil JP; Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States of America., Caposio P; Vaccine and Gene Therapy Institute, Oregon Health Science University, Beaverton, Oregon, United States of America., Padi M; Bioinformatics Shared Resource, Arizona Cancer Center, University of Arizona, Tucson, Arizona, United States of America.; University of Arizona Cancer Center, University of Arizona, Tucson, Arizona, United States of America.; Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, United States of America., Goodrum FD; BIO5 Institute, University of Arizona, Tucson, Arizona, United States of America.; Department of Immunobiology, University of Arizona, Tucson, Arizona, United States of America.; University of Arizona Cancer Center, University of Arizona, Tucson, Arizona, United States of America.; Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, United States of America.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 21. Date of Electronic Publication: 2024 May 21.
DOI:	10.1101/2024.05.21.594597
Abstrakt:	HCMV genes UL135 and UL138 play opposing roles regulating latency and reactivation in CD34 + human progenitor cells (HPCs). Using the THP-1 cell line model for latency and reactivation, we designed an RNA sequencing study to compare the transcriptional profile of HCMV infection in the presence and absence of these genes. The loss of UL138 results in elevated levels of viral gene expression and increased differentiation of cell populations that support HCMV gene expression and genome synthesis. The loss of UL135 results in diminished viral gene expression during an initial burst that occurs as latency is established and no expression of eleven viral genes from the UL b ' region even following stimulation for differentiation and reactivation. Transcriptional network analysis revealed host transcription factors with potential to regulate the UL b ' genes in coordination with pUL135. These results reveal roles for UL135 and UL138 in regulation of viral gene expression and potentially hematopoietic differentiation. Competing Interests: Declaration of Interests: The authors declare no competing interests.
Databáze:	MEDLINE
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