Microfluidic Ecology Unravels the Genetic and Ecological Drivers of T4r Bacteriophage Resistance in E. coli: Insights into Biofilm-Mediated Evolution.

Autor:	Nagy K; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary., Valappil SK; Department of Biotechnology, University of Szeged, Szeged, Hungary., Phan TV; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA., Li S; Department of Physics, Princeton University, Princeton, NJ, USA., Dér L; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary., Morris R; School of Physics & Astronomy, University of Edinburgh, Edinburgh, Scotland., Bos J; Institut Pasteur, Université Paris Cité, CNRS UMR 3525, Unité Plasticité du Génome Bactérien, Paris, France., Winslow S; University of Northwestern St. Paul, Roseville, MN, USA., Galajda P; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary., Ràkhely G; Institute of Biophysics, HUN-REN Biological Research Centre, Szeged, Hungary.; Department of Biotechnology, University of Szeged, Szeged, Hungary., Austin RH; Department of Physics, Princeton University, Princeton, NJ, USA.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2024 May 24. Date of Electronic Publication: 2024 May 24.
DOI:	10.21203/rs.3.rs-4356333/v1
Abstrakt:	We use a microfluidic ecology which generates non-uniform phage concentration gradients and micro-ecological niches to reveal the importance of time, spatial population structure and collective population dynamics in the de novo evolution of T4r bacteriophage resistant motile E. coli . An insensitive bacterial population against T4r phage occurs within 20 hours in small interconnected population niches created by a gradient of phage virions, driven by evolution in transient biofilm patches. Sequencing of the resistant bacteria reveals mutations at the receptor site of bacteriophage T4r as expected but also in genes associated with biofilm formation and surface adhesion, supporting the hypothesis that evolution within transient biofilms drives de novo phage resistance. Competing Interests: Additional Declarations: There is NO Competing Interest.
Databáze:	MEDLINE
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