Electrochemical Monitoring of Real-Time Vesicle Dynamics Induced by Tau in a Confined Nanopipette.

Autor: Chen KL; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails., Yu RJ; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails., Zhong CB; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails., Wang Z; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China., Xie BK; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails., Ma H; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails., Ao M; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China., Zheng P; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China., Ewing AG; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, 41296, Sweden., Long YT; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2024 Sep 23; Vol. 63 (39), pp. e202406677. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1002/anie.202406677
Abstrakt: The microtubule-associated protein tau participates in neurotransmission regulation via its interaction with synaptic vesicles (SVs). The precise nature and mechanics of tau's engagement with SVs, especially regarding alterations in vesicle dynamics, remain a matter of discussion. We report an electrochemical method using a synapse-mimicking nanopipette to monitor vesicle dynamics induced by tau. A model vesicle of ~30 nm is confined within a lipid-modified nanopipette orifice with a comparable diameter to mimic the synaptic lipid environment. Both tau and phosphorylated tau (p-tau) present two-state dynamic behavior in this biomimetic system, showing typical ionic current oscillation, induced by lipid-tau interaction. The results indicate that p-tau has a stronger affinity to the lipid vesicles in the confined environment, blocking the vesicle movement to a higher degree. Taken together, this method bridges a gap for sensing synaptic vesicle dynamics in a confined lipid environment, mimicking vesicle movement near the synaptic membrane. These findings contribute to understanding how different types of tau protein regulate synaptic vesicle motility and to underlying its functional and pathological behaviours in disease.
(© 2024 Wiley-VCH GmbH.)
Databáze: MEDLINE
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