Mechanical Characterization of Vial Strain During Freezing and Thawing Operations Using Amorphous Excipients.

Autor:	Strongrich A; Department of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: astrongr@purdue.edu., Flynn I; Department of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA., Bhatnagar B; BioTherapeutics Pharmaceutical Sciences, Pfizer Inc., Andover, MA, USA., Shalaev E; Development Sciences, R&D, AbbVie Inc., Irvine, CA, USA., Tchessalov S; BioTherapeutics Pharmaceutical Sciences, Pfizer Inc., Andover, MA, USA.
Jazyk:	angličtina
Zdroj:	Journal of pharmaceutical sciences [J Pharm Sci] 2024 Sep; Vol. 113 (9), pp. 2699-2707. Date of Electronic Publication: 2024 May 31.
DOI:	10.1016/j.xphs.2024.05.029
Abstrakt:	The purpose of this study was to investigate the mechanical stresses and strains acting on pharmaceutical glass tubing vials during freezing and thawing of model pharmaceutical formulations. Strain measurements were conducted inside of a laboratory-scale freeze-dryer using a custom wireless sensor. In both sucrose and trehalose formulations at concentrations between 5 % and 20 % w/v, the strain measurements initially increased before peaking in magnitude at temperatures close to the respective glass transition temperatures of the maximally freeze concentrated solutes, Tg'. We attribute this behavior to a shift in the mechanical properties of the frozen system from a purely elastic glass below Tg' to a viscoelastic rubber-like material above Tg'. That is, when the interstitial region becomes mechanically compliant at temperature above Tg'. The outputs were less predictable below 5 % w/v and tended to exhibit two separate peaks in strain output, one near the equilibrium melting temperature of pure ice and the other near Tg'. The peaks merged at concentrations between 4 and 5 % w/v where the largest strain magnitude was observed. The strain on primary packaging has traditionally been applied to evaluate the risk of damage or breakage due to, for example, crystallization of excipients. However, data collected during this study suggest there may be utility in formulation design or as a process analytical technology to minimize potentially destabilizing stresses and strains in the frozen formulation. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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