Comparison of α and β-acid isomerization in hops and beer using HPLC, confocal microscopy, spectrofluorimetry and chemical analysis of metabolites and essential oils in flowers of different hop cultivars produced in Brazil.

Autor: do Nascimento FMG; Programa de Pós-graduação em Química. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970, Fortaleza-CE, Brazil., Trevisan MTS; Programa de Pós-graduação em Química. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970, Fortaleza-CE, Brazil. Electronic address: trevisan@ufc.br., Neto MLA; Departamento de Física, Universidade Federal do Ceará, Campus do Pici, CP: 60440-900, Fortaleza-CE, Brazil. Electronic address: manoellourenco@fisica.ufc.br., Owen RW; Programa de Pós-graduação em Química. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970, Fortaleza-CE, Brazil., de Brito ES; Embrapa Alimentos e Territórios, CP: 57020-050, Maceió-AL, Brazil. Electronic address: edy.brito@embrapa.br., Alexandre E Silva LM; Embrapa Agroindústria Tropical, CP: 60511-110, Fortaleza-CE, Brazil; Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas, Instituto de Biologia, Campinas - SP, Brazil. Electronic address: lorena.mara@embrapa.br., Rocha KAD; Embrapa Agroindústria Tropical, CP: 60511-110, Fortaleza-CE, Brazil. Electronic address: kesya.rocha@colaborador.embrapa.br., Cesar CL; Departamento de Física, Universidade Federal do Ceará, Campus do Pici, CP: 60440-900, Fortaleza-CE, Brazil. Electronic address: lenz@fisica.ufc.br., de Carvalho HF; Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas, Instituto de Biologia, Campinas - SP, Brazil; Instituto Nacional de Ciência e Tecnologia de Fotônica Aplicada à Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas, Campinas - SP, Brazil. Electronic address: hern@unicamp.br., Pelegati VB; Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas, Instituto de Biologia, Campinas - SP, Brazil; Instituto Nacional de Ciência e Tecnologia de Fotônica Aplicada à Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas, Campinas - SP, Brazil. Electronic address: pelegati@unicamp.br., da Silva SA; Departamento de Ensino, Instituto Federal de Educação, Ciência e Tecnologia do Ceará, 63902-580, Quixadá - CE, Brazil., Marques SPD; Programa de Pós-graduação em Química. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970, Fortaleza-CE, Brazil; Instituto Federal de Educação, Ciência e Tecnologia do Ceará. Departamento de Ensino, Rod. CE-040, Km 137,1 s/n, Aracati-CE 62800-000, Brazil. Electronic address: samuel.marques@ifce.edu.br.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Oct 15; Vol. 455, pp. 139879. Date of Electronic Publication: 2024 May 28.
DOI: 10.1016/j.foodchem.2024.139879
Abstrakt: We used confocal microscopy and spectrofluorescence to characterize the emission spectra in hop flowers, to follow the isomerization processes in different hop preparations, and beers, to compare with HPLC extracted samples. Flowers of different hop cultivars produced in three regions of Brazil, were quantitated by HPLC and GC-MS. The fluorescence spectra showed two characteristic emission bands evaluated from different preparations. The isomerization process leads to a gradual decrease in fluorescence intensity as the reaction progresses. This demonstrates the valuable use of confocal microscopy and fluorescence spectroscopy for analysis of the correlation between bitter acid indices with fluorescence intensity and lifetime microscopy. Such techniques can be used directly in the flowers allowing rapid monitoring of the brewing process. Twenty-nine substances were characterized in the essential oils and some cultivars presented quantities of bitter acids and essential oil levels close to those expected for plants after more than three years of cultivation.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Samuel pedro dantas marques reports financial support was provided by Foundation for Scientific and Technological Development and Support of Ceará. Samuel pedro dantas marques reports a relationship with Foundation for Scientific and Technological Development and Support of Ceará that includes: funding grants. Samuel pedro dantas marques reports a relationship with National Council for Scientific and Technological Development that includes: funding grants. The authors have no conflicts of interest to declare that are relevant to the content of this article. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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