Effect of exogenous sperm capacitation inducers on stallion sperm.

Autor: Fuentes F; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile; Doctoral Program in Applied Cellular and Molecular Biology, Universidad de La Frontera, Temuco, Chile., Contreras MJ; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile., Arroyo-Salvo C; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile., Cabrera P P; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile., Silva M; Department of Veterinary Sciences and Public Health, Universidad Catolica de Temuco, Temuco, Chile., Merino O; Department of Basic Sciences, Faculty of Medicine. Universidad de La Frontera, Temuco, Chile., Arias ME; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile; Department of Agricultural Production, Faculty of Agriculture and Environmental Sciences, Universidad de La Frontera, Temuco, Chile., Felmer R; Laboratory of Reproduction, Center of Reproductive Biotechnology (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile; Department of Agricultural Sciences and Natural Resources, Faculty of Agriculture and Environmental Sciences, Universidad de La Frontera, Temuco, Chile. Electronic address: ricardo.felmer@ufrontera.cl.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Sep 15; Vol. 226, pp. 29-38. Date of Electronic Publication: 2024 May 29.
DOI: 10.1016/j.theriogenology.2024.05.042
Abstrakt: Although under appropriate laboratory conditions, sperm from different mammalian species can be capacitated in vitro, the optimal conditions for sperm capacitation in the stallion have been elusive. This study evaluated the effect of different capacitating inducers in Whitten and Tyrode media and assessed their impact on capacitation-related factors. Stallion sperm were incubated with different combinations of capacitating inducers at 38.5 °C in an air atmosphere. Sperm quality variables such as motility, mitochondrial membrane potential, and lipid peroxidation were assessed. Membrane fluidity and intracellular calcium levels were evaluated as early markers of capacitation, while tyrosine phosphorylation events and the sperm's ability to perform acrosomal exocytosis were used as late capacitation markers. Finally, these sperm were evaluated using a heterologous zona pellucida binding assay. The findings confirm that capacitating conditions evaluated increase intracellular calcium levels and membrane fluidity in both media. Similarly, including 2 or 3 inducers in both media increased tyrosine phosphorylation levels and acrosomal exocytosis after exposure to progesterone, confirming that stallion sperm incubated in these conditions shows cellular and molecular changes consistent with sperm capacitation. Furthermore, the zona pellucida binding assay confirmed the binding capacity of sperm incubated in capacitation conditions, a key step for stallion in vitro fertilization success. Further studies are needed to evaluate the effect of these conditions on in vitro fertilization in the horse.
Competing Interests: Declaration of competing interest None of the authors have any conflict of interest to declare.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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