Dynamic Covalent Hydrogel as a Single-Dose Vaccine Adjuvant for Sustained Antigen Release and Significantly Elevated Humoral Immunity.

Autor: Shi Z; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China.; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China., Gao Z; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Zhuang X; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Si X; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; Jilin Biomedical Polymers Engineering Laboratory, Changchun, 130022, China., Huang Z; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China.; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China., Di Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Ma S; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; Jilin Biomedical Polymers Engineering Laboratory, Changchun, 130022, China., Guo Z; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; Jilin Biomedical Polymers Engineering Laboratory, Changchun, 130022, China., Li C; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Jin N; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Huang L; Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, North Carolina, 27599, USA., Tian M; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China., Song W; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China.; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; Jilin Biomedical Polymers Engineering Laboratory, Changchun, 130022, China., Chen X; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China.; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; Jilin Biomedical Polymers Engineering Laboratory, Changchun, 130022, China.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Sep; Vol. 13 (23), pp. e2400886. Date of Electronic Publication: 2024 Jun 20.
DOI: 10.1002/adhm.202400886
Abstrakt: Vaccine is the most important way for fighting against infection diseases. However, multiple injections and unsatisfied immune responses are the main obstacles for current vaccine application. Herein, a dynamic covalent hydrogel (DCH) is used as a single-dose vaccine adjuvant for eliciting robust and sustained humoral immunity. By adjusting the mass ratio of the DCH gel, 10-30 d constant release of the loaded recombinant protein antigens is successfully realized, and it is proved that sustained release of antigens can significantly improve the vaccine efficacy. When loading SARS-CoV-2 RBD (Wuhan and Omicron BA.1 strains) antigens into this DCH gel, an over 32 000 times and 8000 times improvement is observed in antigen-specific antibody titers compared to conventional Aluminum adjuvanted vaccines. The universality of this DCH gel adjuvant is confirmed in a Nipah G antigen test as well as a H1N1 influenza virus antigen test, with much improved protection of C57BL/6 mice against H1N1 virus infection than conventional Aluminum adjuvanted vaccines. This sustainably released, single-dose DCH gel adjuvant provides a new promising option for designing next-generation infection vaccines.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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