Formulation of quinoa oil-alginate loaded nanoemulsion and its anticancer efficacy as a therapy for chemically induced breast cancer.

Autor: El Makawy AI; Cell Biology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt. aelmakawy@yahoo.com., Mabrouk DM; Cell Biology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., Ibrahim FM; Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., Abdel-Aziem SH; Cell Biology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., El-Kader HAMA; Cell Biology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., Youssef DA; Pests and plant protection Department, Agricultural and Biology Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., Sharaf HA; Pathology Department, Medicine and Clinical Studies Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt., Mohammed SE; Food Sciences and Nutrition Department, Food Industries and Nutrition Research institute, National Research Centre, 33 El Bohouth St., Dokki, Giza, P. O. 12622, Egypt.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Jun 01; Vol. 51 (1), pp. 705. Date of Electronic Publication: 2024 Jun 01.
DOI: 10.1007/s11033-024-09619-x
Abstrakt: Background: Quinoa seeds (Chenopodium quinoa Willd.) have gained interest due to their naturally occurring phytochemicals and antioxidants. They possess potent anticancer properties against human colorectal cancer.
Methods and Results: Fatty acids in quinoa oil were studied using gas chromatography-mass spectrometry. Rats were used to test the acute oral toxicity of the nanoemulsion loaded with sodium alginate. The DPPH radical scavenging method was employed to assess the nanoemulsion's ability to scavenge free radicals. It was examined the in vivo anticancer potential of quinoa oil nanoemulsion on rats with breast cancer induced by 7, 12-dimethylbenz (a) anthracene (DMBA). DMBA-breast cancer models received daily quinoa oil nanoemulsions for 30 days. The anticancer effect of the nanoemulsion was assessed by measuring ROS, protein carbonyl, gene expression of anti-oncogenes, and histopathological analysis. Supplying quinoa oil nanoemulsion significantly reduced the increase in serum ROS and PC levels induced in breast cancer tissue. The expression levels of antioncogenes in breast cancer tissue were decreased by the quinoa oil nanoemulsion. Nanoemulsions also improved the cellular morphology of breast tumors.
Conclusion: The study results indicate that quinoa oil nanoemulsion has anticancer activity against breast cancer, effectively modulating oxidative stress markers, anti-oncogene expressions, and tissue architecture. It can be inferred from the results that quinoa oil nanoemulsion is a chemoprotective medication that may hinder breast cancer progression in rats.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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