The study of galactomannans with different molecular weights and their ability to form microparticles suitable for pulmonary delivery.

Autor: Galrinho MF; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Silva LM; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: lisete.silva@ua.pt., Lopes GR; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Ferreira BAC; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Valente SA; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Ferreira I; Centro de Neurociências e Biologia Celular e Centro de Inovação em Biomedicina e Biotecnologia, Universidade de Coimbra, Azinhaga de Santa Comba, 3004-517 Coimbra, Portugal; Faculdade de Farmácia da Universidade de Coimbra, Universidade de Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal., Pinheiro BA; UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry/Department of Life Sciences, School of Science and Technology, NOVA University of Lisbon, 2829-516 Lisbon, Portugal; Associate Laboratory i4HB, Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516, Caparica, Portugal., Palma AS; UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry/Department of Life Sciences, School of Science and Technology, NOVA University of Lisbon, 2829-516 Lisbon, Portugal; Associate Laboratory i4HB, Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516, Caparica, Portugal., Evtuguin DV; CICECO, Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Lopes da Silva JA; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Almeida M; CICECO, Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193 Aveiro, Portugal., Ferreira P; CICECO, Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, 3810-193 Aveiro, Portugal., Cruz MT; Centro de Neurociências e Biologia Celular e Centro de Inovação em Biomedicina e Biotecnologia, Universidade de Coimbra, Azinhaga de Santa Comba, 3004-517 Coimbra, Portugal; Faculdade de Farmácia da Universidade de Coimbra, Universidade de Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal., Coimbra MA; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Passos CP; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: cpassos@ua.pt.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2024 Sep 01; Vol. 339, pp. 122268. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.carbpol.2024.122268
Abstrakt: The influence of locust bean gum (LBG) galactomannans (GMs) molecular weight (Mw) to assemble microparticulate systems was evaluated, and carriers for deep lung delivery were developed. A commercial batch of LBG with a mannose/galactose (M/G) ratio of 2.4 (batch 1) was used to study the influence of different microwave partial acid hydrolysis conditions on carbohydrate composition, glycosidic linkages, and aqueous solutions viscosity. The microwave treatment did not affect the composition, presenting 4-Man (36-42 %), 4,6-Man (27-35 %), and T-Gal (24-25 %) as the main glycosidic linkages. Depolymerization led to a viscosity reduction (≤0.005 Pa·s) with no major impact on polysaccharide debranching. The structural composition of the LBG galactomannans were further elucidated with sequence-specific proteins using carbohydrate microarray technologies. A second batch of LBG (M/G 3.3) was used to study the impact of GMs with different Mw on microparticle assembling, characteristics, and insulin release kinetics. The low-Mw GMs microparticles led to a faster release (20 min) than the higher-Mw (40 min) ones, impacting the release kinetics. All microparticles exhibited a safety profile to cells of the respiratory tract. However, only the higher-Mw GMs allowed the assembly of microparticles with sizes suitable for this type of administration.
Competing Interests: Declaration of competing interest The authors declare that there is no potential conflict of interest.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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