| Autor: |
A Hashim A; Department of Pharmaceutics, College of Pharmacy, Ahl AL-Bayt University, Karbala, Iraq., Altememy D; Department of Pharmaceutics, College of Pharmacy, Al-Zahraa University for Women, Karbala, Iraq., H Mahdi M; Department of Pharmaceutics, College of Pharmacy, Ahl AL-Bayt University, Karbala, Iraq., Salmani S; Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran., Parvizpour S; Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran., Alizadeh A; Nervous System Stem Cells Research Center and Department of Tissue Engineering and Applied Cell Sciences, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran., Ghasemi S; Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. |
| Abstrakt: |
Gastric cancer remains a global health concern, driving the exploration of natural products with anticancer potential. This study investigated the antiproliferative activity and chemical composition of a 70% ethanolic extract from Melissa officinalis L. against human gastric cancer cells. The extract was prepared and evaluated for total phenolic content, antioxidant capacity and flavonoid content. The MTT test checked how well it stopped the growth of human gastric adenocarcinoma (AGS) and normal dermal fibroblast (HDF) cells. Data analysis (SPSS Statistics) determined viable cell percentages and performed regression analysis (p<0.05). The extract exhibited significant antiproliferative activity against AGS cells compared to normal cells (p<0.05), with decreasing IC 50 values (564.3, 258.0 and 122.5 μg/ml) over 24, 48 and 72 hours. It also displayed antioxidant activity (IC50=16.8±1.41μg/ml) and contained substantial phenolics (225.76±4.1 mg GAE/g) and flavonoids (22.36±2.6 mg RUT/g). This study suggests the 70% ethanolic extract of M. officinalis effectively suppresses AGS cell growth and possesses promising antioxidant properties, highlighting its potential as a natural source of anticancer and antioxidant agents, deserving further investigation. |
