Enhancing high-efficiency breeding and microbial microdroplet cultivation techniques for Ganoderma lucidum.

Autor: Feng J; Institute of Edible Fungi, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture of P. R. China, Shanghai, 201403, China., Tang CM; Institute of Edible Fungi, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture of P. R. China, Shanghai, 201403, China., Liu YF; Institute of Edible Fungi, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture of P. R. China, Shanghai, 201403, China., Tang CH; Institute of Edible Fungi, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture of P. R. China, Shanghai, 201403, China., Zhang JS; Institute of Edible Fungi, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture of P. R. China, Shanghai, 201403, China. syja16@saas.sh.cn.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 Jun 01; Vol. 40 (7), pp. 225. Date of Electronic Publication: 2024 Jun 01.
DOI: 10.1007/s11274-024-04011-0
Abstrakt: Ganoderma lucidum is known for its bioactive compounds, such as polysaccharides and triterpenoids, which are crucial in food and medicine. However, liquid fermentation encounters challenges in terms of strain differentiation and stability. In this research, we employed atmospheric room temperature plasma mutation and a microbial microdroplet culture system to identify strains with enhanced biomass and triterpenoid production. The three mutant strains, YB05, YB09, and YB18, exhibited accelerated growth rates and antagonized the initial strain G0023 more effectively than the controls. Notably, YB18 displayed the fastest growth, with a 17.25% increase in colony radius. Shake flask cultivation demonstrated that, compared with the initial strain, YB05 and YB18 had 26.33% and 17.85% greater biomass, respectively. Moreover, the triterpenoid production of YB05 and YB18 surpassed that of the control by 32.10% and 15.72%, respectively, as confirmed by colorimetric detection. Importantly, these mutant strains remained stable for five generations. This study revealed a comprehensive screening system utilizing atmospheric pressure, room temperature plasma mutation technology and microbial droplet cultivation. This innovative approach offers a promising pathway for obtaining advantageous Ganoderma strains for liquid fermentation. The methodology of atmospheric room temperature plasma mutation and microbial microdroplet culture systems is detailed for better comprehension.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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