Extrafibrillar demineralization: Yes or no?

Autor: Zhou Q; Department of Stomatology, The First Affiliated Hospital of Anhui Medical University, 218 Jixilu Rd., Hefei 230032, China; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China., Li Y; Department of Stomatology, The First Affiliated Hospital of Anhui Medical University, 218 Jixilu Rd., Hefei 230032, China; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China., Xiao T; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China., Zhong Q; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China., Wu L; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China., Zhou Z; School of Dentistry, University of Detroit Mercy, Detroit, MI 48208, USA., Wong H; Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, The Prince Philip Dental Hospital, 999077, Hong Kong, SAR China., Li QL; Department of Stomatology, The First Affiliated Hospital of Anhui Medical University, 218 Jixilu Rd., Hefei 230032, China; Key Lab. of Oral Diseases Research of Anhui Province, College & Hospital of Stomatology, Anhui Medical University, 81 Meishan Road, Hefei 230032, China; Department of Stomatology. Longgang Otorhinolaryngology Hospital of Shenzhen, No. 3004L Longgang Avenue, 518172, China. Electronic address: ql-li@126.com.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2024 Aug; Vol. 40 (8), pp. 1113-1122. Date of Electronic Publication: 2024 May 30.
DOI: 10.1016/j.dental.2024.05.017
Abstrakt: Objective: Extrafibrillar demineralization is considered to be an ideal solution for addressing the durability of resin-dentin bonding interfaces. However, its theoretical basis is contradictory to ionization equilibrium of hydroxyapatite dissolution. In this study, various calcium chelators were selected as dentin conditioners to explore the essence of dentin demineralization with chelators and its effect on resin-dentin adhesion.
Methods: Polyethyleneimine grafted with EDTA and polyacrylic acid sodium (PAAN 450k ) larger than 40 kDa, as well as PAAN (PAAN 3k ) and EDTA smaller than 6 kDa, were prepared as dentin conditioners. The dentin powder was designed to characterize whether it would demineralize without contact with PAAN 450k . Dentin demineralization effect with four conditioners was evaluated with field emission scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, atomic force microscopy and quantification of hydroxyproline concentration after enzymatic degradation. Micro-tensile bond strength (µTBS) test and failure mode analysis were employed to assess the bonding effect of the four chelators in both wet and dry bonding, with H 3 PO 4 wet bonding serving as the control group.
Results: Demineralization occurs when PAAN 450k was not in direct contact with the dentin powder. The extrafibrillar demineralization cannot be induced by any chelator regardless of its molecular weight. Complete demineralization including extrafibrillar and intrafibrillar demineralization would occur with sufficient interaction time. Moreover, chelators could not provide a reliable dentin bonding effect under a short interaction time.
Significance: From the perspective of theory and application, extrafibrillar demineralization is not a reliable strategy, which provides a reminder for exploring new strategies in the future.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE