Characterization of the m 6 A gene family in switchgrass and functional analysis of PvALKBH10 during flowering.

Autor: Liu H; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Lin M; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Zhou D; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Liu B; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Li X; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Wang H; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China., Bi X; College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China. Electronic address: xiaojing.bi@cau.edu.cn.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Jul; Vol. 212, pp. 108781. Date of Electronic Publication: 2024 May 29.
DOI: 10.1016/j.plaphy.2024.108781
Abstrakt: N 6 -methyladenosine (m 6 A), a nucleotide modification that is frequently seen in RNA, plays a crucial role in plant growth, development and stress resistance. However, the m 6 A regulatory machinery in switchgrass (Panicum virgatum L.), a model plant for cellulose-to-ethanol conversion, remains largely unknown. In this study, we identified 57 candidate genes involved in m 6 A-regulation in the switchgrass genome, and analyzed their chromosomal distribution, evolutionary relationships, and functions. Notably, we observed distinct gene expression patterns under salt and drought stress, with salt stress inducing writer and eraser genes, alongside drought stress predominantly affecting reader genes. Additionally, we knocked out PvALKBH10, an m 6 A demethylase gene, via CRISPR/Cas9 and found its potential function in controlling flowering time. This study provides insight into the genomic organization and evolutionary features of m 6 A-associated putative genes in switchgrass, and therefore serves as the basis for further functional studies.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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