Autonomous assembly and disassembly of gliding molecular robots regulated by a DNA-based molecular controller.

Autor:	Kawamata I; Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan., Nishiyama K; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan., Matsumoto D; Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan., Ichiseki S; Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan., Keya JJ; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan., Okuyama K; Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan., Ichikawa M; Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan., Kabir AMR; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan., Sato Y; Department of Intelligent and Control Systems, Kyushu Institute of Technology, Iizuka 820-8502, Japan., Inoue D; Faculty of Design, Kyushu University, Fukuoka 815-8540, Japan., Murata S; Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan., Sada K; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan.; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan., Kakugo A; Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan., Nomura SM; Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2024 May 31; Vol. 10 (22), pp. eadn4490. Date of Electronic Publication: 2024 May 31.
DOI:	10.1126/sciadv.adn4490
Abstrakt:	In recent years, there has been a growing interest in engineering dynamic and autonomous systems with robotic functionalities using biomolecules. Specifically, the ability of molecular motors to convert chemical energy to mechanical forces and the programmability of DNA are regarded as promising components for these systems. However, current systems rely on the manual addition of external stimuli, limiting the potential for autonomous molecular systems. Here, we show that DNA-based cascade reactions can act as a molecular controller that drives the autonomous assembly and disassembly of DNA-functionalized microtubules propelled by kinesins. The DNA controller is designed to produce two different DNA strands that program the interaction between the microtubules. The gliding microtubules integrated with the controller autonomously assemble to bundle-like structures and disassemble into discrete filaments without external stimuli, which is observable by fluorescence microscopy. We believe this approach to be a starting point toward more autonomous behavior of motor protein-based multicomponent systems with robotic functionalities.
Databáze:	MEDLINE
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