From mec cassette to rdhA : a key Dehalobacter genomic neighborhood in a chloroform and dichloromethane-transforming microbial consortium.
Autor: | Bulka O; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada., Picott K; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada., Mahadevan R; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada., Edwards EA; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.; Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada. |
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Jazyk: | angličtina |
Zdroj: | Applied and environmental microbiology [Appl Environ Microbiol] 2024 Jun 18; Vol. 90 (6), pp. e0073224. Date of Electronic Publication: 2024 May 31. |
DOI: | 10.1128/aem.00732-24 |
Abstrakt: | Chloroform (CF) and dichloromethane (DCM) are groundwater contaminants of concern due to their high toxicity and inhibition of important biogeochemical processes such as methanogenesis. Anaerobic biotransformation of CF and DCM has been well documented but typically independently of one another. CF is the electron acceptor for certain organohalide-respiring bacteria that use reductive dehalogenases (RDases) to dechlorinate CF to DCM. In contrast, known DCM degraders use DCM as their electron donor, which is oxidized using a series of methyltransferases and associated proteins encoded by the mec cassette to facilitate the entry of DCM to the Wood-Ljungdahl pathway. The SC05 culture is an enrichment culture sold commercially for bioaugmentation, which transforms CF via DCM to CO Importance: Chloroform (CF) and dichloromethane (DCM) are regulated groundwater contaminants. A cost-effective approach to remove these pollutants from contaminated groundwater is to employ microbes that transform CF and DCM as part of their metabolism, thus depleting the contamination as the microbes continue to grow. In this work, we investigate bioaugmentation culture SC05, a mixed microbial consortium that effectively and simultaneously degrades both CF and DCM coupled to the growth of Dehalobacter . We identified the functional genes responsible for the transformation of CF and DCM in SC05. These genetic biomarkers provide a means to monitor the remediation process in the field. Competing Interests: The authors declare no conflict of interest. |
Databáze: | MEDLINE |
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