Bartonella species in dromedaries and ruminants from Lower Shabelle and Benadir regions, Somalia.

Autor: Osman AM; Graduate Program on Veterinary Sciences, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.; Somali One Health Centre, Abrar University, Mogadishu, Somalia.; Department of Animal Health and Veterinary Services, Ministry of Livestock, Forestry, and Range, Mogadishu, Somalia., Hassan-Kadle AA; Somali One Health Centre, Abrar University, Mogadishu, Somalia.; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia., Dias CM; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal, Brazil., Ibrahim AM; Graduate Program on Veterinary Sciences, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.; Somali One Health Centre, Abrar University, Mogadishu, Somalia.; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia., Collere FCM; Graduate Program on Veterinary Sciences, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., Shair MA; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia., Montiani-Ferreira F; Graduate Program on Veterinary Sciences, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.; Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., André MR; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal, Brazil., Yusuf AA; Abrar Research and Training Centre, Abrar University, Mogadishu, Somalia.; Department of Slaughterhouses, Somali Meat Company, Mogadishu, Somalia., Vieira TSWJ; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, North Carolina, USA., Machado RZ; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal, Brazil., Vieira RFC; Center for Computational Intelligence to Predict Health and Environmental Risks (CIPHER), The University of North Carolina at Charlotte, Charlotte, North Carolina, USA.; Department of Epidemiology and Community Health, The University of North Carolina at Charlotte, Charlotte, North Carolina, USA.
Jazyk: angličtina
Zdroj: Zoonoses and public health [Zoonoses Public Health] 2024 Aug; Vol. 71 (5), pp. 568-577. Date of Electronic Publication: 2024 May 30.
DOI: 10.1111/zph.13158
Abstrakt: Background: Bartonellosis, caused by bacteria of the genus Bartonella, is a zoonotic disease with several mammalian reservoir hosts. In Somalia, a country heavily reliant on livestock, zoonotic diseases pose significant public health and economic challenges. To the best of our knowledge, no study has been performed aiming to verify the occurrence of Bartonella spp. in Somalia. This study investigated the occurrence and molecular characterization of Bartonella in dromedary (Camelus dromedarius, Linnaeus, 1758), cattle, sheep, and goats from Somalia.
Materials and Methods: 530 blood samples were collected from various animals (155 dromedary, 199 goat, 131 cattle, and 45 sheep) in Benadir and Lower Shabelle regions. DNA was extracted for molecular analysis, and a qPCR assay targeting the NADH dehydrogenase gamma subunit (nuoG) gene was used for Bartonella screening. Positive samples were also subjected to PCR assays targeting seven molecular markers including: nuoG, citrate synthase gene (gltA), RNA polymerase beta-subunit gene (rpoB), riboflavin synthase gene (ribC), 60 kDa heat-shock protein gene (groEL), cell division protein gene (ftsZ), and pap31 and qPCR targeting the 16-23S rRNA internal transcribed spacer (ITS) followed by Sanger sequencing, BLASTn and phylogenetic analysis.
Results: Out of 530 tested animals, 5.1% were positive for Bartonella spp. by the nuoG qPCR assay. Goats showed the highest Bartonella occurrence (17/199, 8.5%), followed by sheep (6/44, 6.8%), cattle (4/131, 3.1%), and dromedary (1/155, 1.9%). Goats, sheep, and cattle had higher odds of infection compared to dromedary. Among nuoG qPCR-positive samples, 11.1%, 14.8%, 11.1%, and 25.9% were positive in PCR assays based on nuoG, gltA, and pap31 genes, and in the qPCR based on the ITS region, respectively. On the other hand, nuoG qPCR-positive samples were negative in the PCR assays targeting the ribC, rpoB, ftsZ, and groEL genes. While Bartonella bovis sequences were detected in cattle (nuoG and ITS) and goats (gltA), Bartonella henselae ITS sequences were detected in dromedary, goat, and sheep. Phylogenetic analysis placed gltA Bartonella sequence from a goat in the same clade of B. bovis.
Conclusion: The present study showed, for the first time, molecular evidence of Bartonella spp. in dromedary and ruminants from Somalia and B. henselae in sheep and goats globally. These findings contribute valuable insights into Bartonella spp. occurrence in Somali livestock, highlighting the need for comprehensive surveillance and control measures under the One Health approach.
(© 2024 The Author(s). Zoonoses and Public Health published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: