Arabidopsis AN3 and OLIGOCELLULA genes link telomere maintenance mechanisms with cell division and expansion control.

Autor: Agabekian IA; Institute of Fundamental Medicine and Biology, Kazan Federal University, Republic of Tatarstan, Kazan, 420008, Russia., Abdulkina LR; Institute of Fundamental Medicine and Biology, Kazan Federal University, Republic of Tatarstan, Kazan, 420008, Russia., Lushnenko AY; Institute of Fundamental Medicine and Biology, Kazan Federal University, Republic of Tatarstan, Kazan, 420008, Russia., Young PG; Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, Texas, 77843-2128, USA., Valeeva LR; Department of Biological Sciences, College of Science, Marshall University, Huntington, West Virginia, 25701, USA., Boskovic O; Department of Biological Sciences, College of Science, Marshall University, Huntington, West Virginia, 25701, USA., Lilly EG; Department of Biological Sciences, College of Science, Marshall University, Huntington, West Virginia, 25701, USA., Sharipova MR; Institute of Fundamental Medicine and Biology, Kazan Federal University, Republic of Tatarstan, Kazan, 420008, Russia., Shippen DE; Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, Texas, 77843-2128, USA. dshippen@tamu.edu., Juenger TE; Department of Integrative Biology, University of Texas at Austin, Austin, Texas, 78712, USA. tjuenger@austin.utexas.edu., Shakirov EV; Department of Biological Sciences, College of Science, Marshall University, Huntington, West Virginia, 25701, USA. shakirov@marshall.edu.; Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, 25755, USA. shakirov@marshall.edu.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2024 May 30; Vol. 114 (3), pp. 65. Date of Electronic Publication: 2024 May 30.
DOI: 10.1007/s11103-024-01457-6
Abstrakt: Telomeres are conserved chromosomal structures necessary for continued cell division and proliferation. In addition to the classical telomerase pathway, multiple other genes including those involved in ribosome metabolism and chromatin modification contribute to telomere length maintenance. We previously reported that Arabidopsis thaliana ribosome biogenesis genes OLI2/NOP2A, OLI5/RPL5A and OLI7/RPL5B have critical roles in telomere length regulation. These three OLIGOCELLULA genes were also shown to function in cell proliferation and expansion control and to genetically interact with the transcriptional co-activator ANGUSTIFOLIA3 (AN3). Here we show that AN3-deficient plants progressively lose telomeric DNA in early homozygous mutant generations, but ultimately establish a new shorter telomere length setpoint by the fifth mutant generation with a telomere length similar to oli2/nop2a -deficient plants. Analysis of double an3 oli2 mutants indicates that the two genes are epistatic for telomere length control. Telomere shortening in an3 and oli mutants is not caused by telomerase inhibition; wild type levels of telomerase activity are detected in all analyzed mutants in vitro. Late generations of an3 and oli mutants are prone to stem cell damage in the root apical meristem, implying that genes regulating telomere length may have conserved functional roles in stem cell maintenance mechanisms. Multiple instances of anaphase fusions in late generations of oli5 and oli7 mutants were observed, highlighting an unexpected effect of ribosome biogenesis factors on chromosome integrity. Overall, our data implicate AN3 transcription coactivator and OLIGOCELLULA proteins in the establishment of telomere length set point in plants and further suggest that multiple regulators with pleiotropic functions can connect telomere biology with cell proliferation and cell expansion pathways.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
Externí odkaz: