Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas.

Autor: Lallemand S; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France., Oyhenart J; Instituto de Ciencias de la Tierra y Ambientales de La Pampa, Consejo Nacional de Investigaciones Científicas y Técnicas - Facultad de Ciencias Naturales Universidad Nacional de La Pampa, La Pampa, Argentina., Valot B; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France., Borne R; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France., Bohard L; Department of Infectiology, University Hospital of Besançon, 25030 Besançon, France., Umhang G; INTERFAS Unit, National Reference Laboratory for Echinococcus spp., Rabies and Wildlife Laboratory, ANSES, Malzéville, France., Karamon J; Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Puławy, Poland., Konyaev S; Institute of Systematics and Ecology of Animals, SB RAS, Novosibirsk, Russia., Rönnberg C; Public Health Agency of Sweden, Department of Microbiology, Unit for Parasitology, Solna, Sweden., Gottstein B; Institute of Infectious Diseases, Faculty of Medicine, University of Bern, CH-3012 Bern, Switzerland., Weil-Verhoeven D; Department of Hepatology and Intensive Cares, University Hospital of Besançon, 25030 Besançon, France., Richou C; Department of Hepatology and Intensive Cares, University Hospital of Besançon, 25030 Besançon, France., Bresson-Hadni S; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France; Department of Parasitology-Mycology, Reference National Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France., Millon L; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France; Department of Parasitology-Mycology, Reference National Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France., Bellanger AP; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France; Department of Parasitology-Mycology, Reference National Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France., Knapp J; UMR CNRS 6249 Chrono-Environnement, University of Franche-Comté, 16 Route de Gray, 25030 Besançon cedex, France; Department of Parasitology-Mycology, Reference National Centre for Echinococcoses, University Hospital of Besançon, 25030 Besançon, France. Electronic address: jknapp@univ-fcomte.fr.
Jazyk: angličtina
Zdroj: International journal for parasitology [Int J Parasitol] 2024 Sep; Vol. 54 (11), pp. 569-582. Date of Electronic Publication: 2024 May 28.
DOI: 10.1016/j.ijpara.2024.05.004
Abstrakt: Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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